RAGE-ROS信号轴在糖尿病性牙周炎牙龈上皮凋亡中的作用

The role of RAGE-ROS axis in the apoptosis of gingival fibroblast in diabetic periodontitis

目的:研究RAGE-ROS信号轴在糖尿病性牙周炎牙龈上皮细胞凋亡发生中的作用。方法:选用6周龄雄性Wistar大鼠30只,随机分为正常对照组(N组)及糖尿病组(D组)。采用一次性腹腔注射链脲佐菌素(STZ)的方法诱导糖尿病大鼠模型。4周后分批处死,检测牙槽骨丧失、牙龈上皮细胞凋亡水平,晚期糖基化终末产物受体(RAGE)及活性氧(ROS)水平。结果:D组牙槽骨丧失明显高于N组,且牙龈上皮细胞凋亡发生明显增加(P<0.05);D组牙龈组织RAGE及ROS表达水平也高于N组(P<0.05)。结论:RAGE-ROS信号轴在糖尿病性牙周炎牙龈上皮细胞的凋亡中发挥着重要作用,为认识糖尿病性牙周炎的发病机制提供了线索。

Objective： To investigate the role of RAGE-ROS axis in the apoptosis of gingival fibroblast in diabetic periodontitis. Methods： Thirty wistar rats（male, six weeks old） were randomly divided into two groups： normal control group（N） and diabetic periodontitis group（D）. Four weeks later, animals were sacrificed; meanwhile, the alveolar bone loss and apoptosis of gingival fibroblast were detected while the expression of RAGE and oxidative stress marker were also investigated. Results： Compared to N group, more severe alveolar bone loss, along with more apoptosis of gingival fibroblast appeared in D group（P〈0.05）. The expression of RAGE and oxidative stress marker increased significantly in gingival tissue in D group（P〈0.05）. Conclusion： RAGEROS axis plays an important role in the apoptosis of gingival fibroblast in diabetic periodontitis.