全血中硒测定的微波消解-原子荧光光谱法

Determination of selenium in whole blood by microwave digestion-atomic fluorescence spectrometry

目的建立一种准确、快速、简便的全血中硒测定的原子荧光光谱方法。方法全血样品经微波消解赶酸后，加入50％盐酸预还原，采用氢化物发生-原子荧光光谱法测定全血中硒的含量。结果硒含量在5．0～60．0μg／L线性关系良好，相关系数r=0．9999，方法检出限为0．133μg／L，相对标准偏差（RsD）为1．22％～2．08％，回收率为96．5％～101．8％。结论该方法操作简单，消耗化学试剂少，污染小，精密度和准确度可以满足全血样品检测要求。

Objective To establish a rapid,simple, and accurate method for measuring selenium in whole blood by atomic fluorescence spectrometry. Methods The whole blood samples were treated by microwave digestion and the acids were dispelled. After that the samples were pretreated with 50% hydrochloric acid as a reducing agent. Then the content of selenium in the pretreated samples was measured by hydride generation atomic fluorescence spectrometry. Results The calibration curve for selenium is linear in the range of 0-60 μg/L （r=0.999 9）. The detection limit, relative standard deviation, and recovery rate were 0.133 μg/L, 1.22%-2.08%, and 96.5%-101.8%, respectively. Conclusion This method is simple, with less consumption of chemical reagents, less pollution, and enough accuracy and sensitivity for determination of selenium in whole blood samples.