裴氏升血颗粒合TP-5对荷H22(肝癌)瘤小鼠免疫功能的影响

Effect of Peishishengxue Electuary with TP-5 on the Immunologic Function of H22 Tumor-bearing Mice

目的:研究裴氏升血颗粒合TP-5对荷H22(肝癌)瘤小鼠免疫功能的影响。方法:本研究拟采用裴氏升血颗粒、裴氏升血颗粒与胸腺五肽联合、胸腺五肽分别对荷H22(肝癌)瘤小鼠进行干预,而后通过对小鼠抑瘤率、胸腺指数、脾脏指数、脾淋巴细胞增值能力、血清IL-2、IL-4水平进行检测及数据处理,了解药物干预后荷H22(肝癌)瘤小鼠的免疫器官状态及免疫功能变化。结果:PSXKL组、PSXKL+TP-5组荷瘤小鼠的结束体重、平均瘤重、抑瘤率、胸腺指数(TI)、脾脏指数(SI)均与模型组有显著差异;TP-5组的胸腺指数(TI)、脾脏指数(SI)均与模型组有显著差异。而PSXKL组、PSXKL+TP-5组荷瘤小鼠的结束体重与TP-5组相比有显著差异。PSXKL+TP-5组和TP-5组荷瘤小鼠的血清IL-2和IL-4含量、脾B淋巴细胞增殖率(%)(以LPS为刺激因子)、脾T淋巴细胞增殖率(%)(以Con A为刺激因子)均与模型组有显著差异。结论:TP-5对荷瘤小鼠免疫器官有明显影响,而对荷瘤小鼠的全身状况无明显改善,使荷瘤小鼠细胞免疫功能增强,对荷瘤小鼠脾T淋巴细胞增殖功能有积极影响。裴氏升血颗粒及其与TP-5联合后均对荷瘤小鼠的全身状况有明显改善,同时对其免疫器官也有明显的影响,使之趋于正常;使荷瘤小鼠细胞免疫功能增强,同时对荷瘤小鼠脾T淋巴细胞、B淋巴细胞增殖功能有积极影响。

Objective： To study the effect of Peishishengxue electuary with TP-5 on the immunologic function of H22 Tumor-bearing Mice. Methods： This study intended to use Peishishengxue electuary, Peishishengxue electuary and TP-5, TP-5 on the intervention of H22 Tumor-bearing Mice. Then tumor inhibition rate, thymus index, spleen index, spleen lymphocyte proliferation ability and serum IL-2, IL-4 level of mice were detected and data processed to investigate the changes of immune organs and immune fimction in H22 Tumor-bearing Mice after drug intervention. Results： The average end weight, tumor weight, tumor inhibition rate, thymus index （TI）, spleen index （SI） of the PSXKL group and the TP-5 PSXKL group were significantly different from that of the model group. The thymus index （TI） and spleen index （SI） of the TP-5 group were significantly different from that of the model group. The end weight of the PSXKL group and the TP-5 PSXKL group were significantly different tYom that of the TP-5 group. The serum levels of IL-2 and IL-4, spleen B lymphocyte proliferation rate （%） （LPS as the stimulating factor）, spleen T lymphocyte proliferation rate （%） （ConA as the stimulating factor） of the TP-5 PSXKL group and TP-5 group were significantly different from that of the model group. Conclusion： TP-5 had a significant effect on the immune organs of H22 Tumor-bearing Mice, and systemic status of H22 Tumor-bearing Mice was not significantly improved, which enhanced the immunity function of cells in mice and had a positive impact on spleen T lymphocyte proliferation function of H22 Tumor-bearing Mice. Peishishengxue electuary combined with TP-5 improved systemic conditions of H22 Tumor-bearing Micesignificantly and showed an obvious effbct on the immune organ, which became normal; it could also enhance the cell immunity fimction of H22 Tumor-bearing Mice and have positive effect on tumor bearing mice spleen T lymphocyte, B lymphocyte proliferation function.