丛枝菌根侵染强度与二年生三七生物量和药效成分含量的相关性研究

Relationship between Intensity of the Mycorrhizal Colonisation in Root System and Biomass or Saponins Content of Panax notoginseng

目的:研究丛枝菌根侵染强度对三七生长和药效成分皂苷的影响;方法:在大田条件下,对三七种子接种丛枝菌根真菌Glomus mosseae,生长二年后,随机采集100株三七,分株测定菌根侵染强度、地下部生物量和皂苷含量;结果:随着丛枝菌根侵染强度的增加,三七地下部生物量、人参皂苷Rd和四种皂苷总含量均出现明显地先增加而后出现下降趋势。同时,随着丛枝菌根侵染强度的增加,三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1、人参皂苷Rd以及这四种皂苷的总产量均出现明显地先增加后下降的趋势。结论:丛枝菌根侵染强度与三七地下部生物量、皂苷含量及皂苷产量存在显著的相关性,具有低侵染增加,高侵染转而降低的现象。丛枝菌根适度侵染时三七有最大的生物量和最高的皂苷含量及皂甙产量。

Objective ： To research the effect of mycorrhizal colonisation on the growth and saponins content of Panax notoginseng. Methods： The effects of AMF on the growth and the saponins content in the root of P. notoginsengwere examined by introducing exogenous Glomus mosseae into two years plants under field condition. Results ： With the increasing of intensity of the mycorrhizal colonization, it showed a trends of increase first and then decrease for the biomass, ginsenoside Rd and total contents of notoginsenoside R1 , ginsenoside Rg1 , ginsenoside Rb1 and ginsenoside Rd in the inoculated plants. Conclusion： There was a strong correlation between the intensity of the mycorrhizal colonization and the plant biomass or contents and total yield of saponins. When the intensity of the mycorrhizal colonization was intermediate range, the biomass, saponins contents and saponins amount reached maximum.