藏药郎庆阿塔对肝纤维化大鼠肝脏TGF-β_1、α-SMA和瘦素受体的影响

Effect of Lang Qing A Ta on TGF-β1,α-SMA and OB-R of Collagenin Rat Hepatic Fibrosis Model

目的:研究藏药郎庆阿塔对肝纤维化大鼠肝脏TGF-β1、α-SMA和瘦素受体的影响。方法:以复合因素造成大鼠肝纤维化模型,模型大鼠经郎庆阿塔干预后,以免疫组化法检测肝组织中TGF-β1和α-SMA的表达水平,以荧光定量PCR(Q-PCR)法检测肝组织瘦素受体mRNA的水平。结果:由免疫组化实验结果发现郎庆阿塔能明显降低肝纤维化大鼠肝组织中TGF-β1和α-SMA的表达水平(P<0.01),Q-PCR结果表明郎庆阿塔能明显降低肝纤维化大鼠肝组织中瘦素受体mRNA的表达。结论:藏药郎庆阿塔具有明显减少肝纤维化大鼠肝脏TGF-β1、α-SMA和瘦素受体表达的作用。

Objective： To study the effect of Lang Qing A Ta（LQAT） on TGF-β1,α-SMA and OB-R of collagen in rat hepatic fibrosis model. Methods： Composite factors were used to induce the hepatic fibrosis models in rats and the model rats were orally treated with corresponding decoctions. The levels of TGF-β1 and α-SMA in livers were measured by immunohistochemisty. The mRNA expression of leptin receptor was detected with real-time Q-PCR. Results： LQAT decoction could remarkably decrease the levels of TGF-β1 and α-SMA in livers of hepatic fibrosis rats. The mRNA expression of OB-R in the rat liver tissues of the LQAT treatment groups was significantly decreased as compared with that of the model group. Conclusion： The therapeutic effect of LQAT decoction on hepatic fibrosis was correlated closely with inhibiting HSC activation and InRNA expression of OB-R in the rat liver tissues.