人EpCAM蛋白胞外区基因原核表达载体的构建及鉴定

Construction and identification of prokaryotic expression vector of human Ep CAM protein extracellular domain gene

目的构建人EpCAM蛋白胞外区基因原核表达载体。方法通过聚合酶链式反应来扩增人EpCAM分子胞外区的编码基因,将目的基因克隆至T载体,通过酶切反应构建pET32a-EpCAM和pcold-TF-EPCAM重组表达载体。结果 EpCAM胞外区基因成功与pET32a和pcold-TF质粒连接。结论本研究通过构建EpCAM胞外区的原核表达载体,为深入研究EpCAM分子的功能提供了实验基础。

Objective To construct prokaryotic expression vectors containing the extracellular domain gene of human Ep CAM. Methods PCR the sequence of human Ep CAM protein extracellular domain gene,the target gene was connected to T vector,and constructed p ET32a-Ep CAM and pcold-TF-EPCAM expression vectors by endonuclease reaction. Results Ep CAM was successfully connected with p ET32 a and pcold-TF plasmids by gene sequencing. Conclusion The construction and identification of human Ep CAM protein extracellular domain gene expression vectors provided basis for its further research.