摘要
目的探索间变性大细胞淋巴瘤(ALCL)小鼠模型的构建方法。方法选取BALB/c小鼠为研究对象,环磷酰胺抑制小鼠机体免疫功能,用Karpas299细胞在小鼠体内建立ALCL模型。结果 1ALCL模型成功建立,免疫组织化学结果显示,CD30和间变性淋巴瘤激酶(ALK)阳性;2聚合酶链反应(PCR)结果显示,核基因重组激活基因2(RAG2)条带阳性,大小517 bp;3环磷酰胺腹腔注射后,小鼠外周血CD3、CD8、CD19和CD20阳性细胞比例显著降低,与空白组比较差异有统计学意义(P<0.05);4成瘤组小鼠外周血CD3、CD8、CD19及CD20阳性细胞比例显著低于未成瘤组小鼠(P<0.05)。结论采用环磷酰胺抑制小鼠机体免疫功能,可成功构建BALB/c小鼠ALCL模型;细胞免疫功能低下可能是模型成功建立的原因或机制。
[Objective] To evaluate the establishment method of anaplastic large cell lymphoma (ALCL) model in mice. [Methods] BALB/c mice were chosen as research subjects. Cyelophosphamide (CTX) was used to inhibit the immune function of mice, and then the ALCL model was established by Karpas 299 sub- cutaneous injection. [Results] ① ALCL model was successfully established. The immunohistoehemistry result showed both CD30 and anaplastic lymphoma kinase (ALK) were positive. ② The PCR result showed that the RAG2 gene was positive and had 517 bp. ③ The percentages of CD3+, CD8+, CD19+ and CD20+ cells after CTX injection were lower than those before injection ((P〈 0.05). ④ The percentages of CD3+, CD8+, CD19+ and CD20+ cells in the tumor group were significantly lower than those in the non-tumor group (P 〈 0.05). [Conclusions] Using eyelophosphamide to inhibit the immune function, ALCL model of BALB/c mice can be successfully established. The low cellular immune function may be the reason or mechanism of successful es- tablishment of ALCL model.
出处
《中国现代医学杂志》
CAS
北大核心
2015年第35期1-6,共6页
China Journal of Modern Medicine
基金
国家自然科学基金(No:81160300)