卡铂诱导的氧化压力对HN-3人喉鳞癌细胞迁徙的影响

The role Played in carboplatin-induced oxidative stress in migration suppression of HN-3 human laryngeal carcinoma cells

目的观察卡铂(carboplatin)对HN-3人喉鳞癌细胞迁徙的影响,探讨活性氧生成在其中的作用。方法用卡铂以不同孵育浓度、不同孵育时间处理HN-3细胞,MTT法测定细胞活力,H2DCFDA染色测定活性氧生成,细胞划痕(Wound healing)评价细胞迁徙,蛋白印迹法(Western blotting)测定表皮生长因子受体(epidermal growth factor receptor,EGFR)表达。结果卡铂诱导HN-3细胞活性氧生成,对体外培养的HN-3细胞增殖活力和迁徙有抑制作用,呈剂量依赖性和时间依赖性;卡铂诱导氧化压力下EGFR表达下调。结论卡铂可抑制人喉鳞癌HN-3细胞增殖与迁徙,其机制可能与卡铂诱导HN-3细胞活性氧生成及氧化压力下EGFR表达下调有关。

Objective To investigate the role played in carboplatin-induced oxidative stress in suppressing cell viability and migration of HN-3 human laryngeal squamous carcinoma cells. Methods The attached HN-3 carcinoma ceils were treated with carboplatin of different concentration and O. 08 mg/ml carboplatin at indicated time points. MrlT assay was used for detecting the cellular viability. H2DCFDA staining was performed to monitor reactive oxygen series （ROS） generation. Wound healing assay was used to for assessing the cell migration; and the Western blot technique for detecting the expression of epidermal growth factor receptor （EGFR）. Results Remarkable ROS generation of HN-3 cells were initiated by carboplatin in dose-related manner. Carboplatin-induced suppression of cellular viability and migration on HN- 3 cells was exhibited in a dose and time related pattern. Down-regulated expression of EGFR under oxidative stress was induced by carboplatin. Conclusion Generation of ROS and down-regulated EGFR expression under oxidative stress may play an important role in the suppression of cellular viability and migration of HN-3 cells exerted by carboolatin.