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TLR4、TBK/IRF3信号通路介导肾小管上皮细胞与单核细胞相互作用 被引量：4

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摘要 目的:探讨单核细胞与肾小管上皮细胞相互作用的分子机制。方法:体外建立单核细胞(U937)与肾小管上皮细胞(HK-2)共培养系统,观察HK-2细胞对U937细胞Toll样受体4(toll-like receptors 4,TLR4)及TANK结合蛋白激酶(TBK)/干扰素调节因子3(IRF3)信号通路的影响;观察阻断TBK/IRF3信号通路对单核细胞分化的影响。结果:HK-2细胞促进U937细胞TLR4及TBK/IRF3信号通路蛋白及mRNA的表达(P<0.05);阻断TBK/IRF3信号通路抑制HK-2细胞诱导U937细胞发生M1型转化(P<0.05)。结论:TLR4分子介导HK-2细胞与U937细胞相互作用,激活TBK/IRF3信号通路,导致U937细胞发生M1型转化,进一步诱导炎症因子产生,促进肾脏纤维化。
作者 李青 郑敏 张露 刘利华 孙伟
机构地区 南京中医药大学附属医院肾内科
出处 《中国中西医结合肾病杂志》 2015年第11期994-996,I0005,共4页 Chinese Journal of Integrated Traditional and Western Nephrology
基金 南京中医药大学青年基金资助项目(No.12XZR11) 国家自然科学基金青年项目(No.81300631) 江苏省自然科学基金青年项目(No.BK20131036)
关键词 单核/巨噬细胞 肾小管上皮细胞 TLR4 TBK/IRF3
分类号 R692 [医药卫生—泌尿科学]
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参考文献10

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同被引文献30

  • 1Obrador GT, Mahdavi-Mazdeh M, Collins AJ. Establishing the global kidney disease prevention network(KDPN) : a posi- tion statement from the National Kidney Foundation[J]. Am J Kidney Dis, 2011, 57(3): 361-370.
  • 2Anders H J, Ryu M. Renal mieroenvironments and macro- phage phenotypes determine progression or resolution of renal inflammation and fibrosis[J]. Kidney Int, 2011, 80(9): 915- 925.
  • 3Alsuwaida AO. Interstitial inflammation and long-term renal outcomes in lupus nephritis[J]. Lupus, 2013, 22(14): 1446- 1454.
  • 4Rieardo SD, Van GH, Eddy AA. Maerophage diversityin re- nal injury and repair[J]. J Clin Invest, 2008, 118(11): 3522 3530.
  • 5Li Q, Liu 1312, Lv LL, et al. Monocytes induce proximal tubu- lar epithelial-mesenehymal transition through NF-kappa B de- pendent upregulation of ICAM-1 [J]. J Cell Bioehem, 2011, 112(6) : 1585-1592.
  • 6Li Q, Lv LL, Wu M, et al. Dexamethasone prevents mono- eyte-indueed tubular epithelial-mesenehymal transition in HK-2 cells[J]. J Cell Bioehem, 2011, 114(3): 632-638.
  • 7Zoja C, Abbate M, Remuzzi G. Progression of renal injury to- ward interstitial inflammation and glomerular sclerosis is de- pendent on abnormal protein filtration [J ]. Nephrol Dial Transplant, 2015, 30(5).. 706-712.
  • 8Altunoglu A, Yavuz D, Canoz MB, et al. Relationship be- tween inflammation and sex hormone profile in female patients receiving different types of renal replacement therapy [J]. Transplant Proc, 2014, 46(5) : 1585-1590.
  • 9Italiani P, Boraschi D. From monocytes to M1/M2 macropha- ges: phenotypical vs functional differentiation[J]. Front Im- munol, 2014, 5(4): 514-535.
  • 10Jo SK, Sung SA, Cho KJ, et al. Macrophages contribute to the initiation of ischaemic acute renal failure in rats[J]. Neph- rol Dial Transplant, 2006, 21(5).. 1231-1239.

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中国中西医结合肾病杂志
2015年 第11期

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