当归补血汤对糖尿病肾病大鼠高密度脂蛋白及微量蛋白尿的影响

Effect of Astragals and Angelica Mixture on the High Density Lipoprotein and Urinary Microalbuminuria in Diabetic Nephropathy Rats

目的:观察当归补血汤对糖尿病大鼠血清高密度脂蛋白(HDL)及尿微量白蛋白(MAU)的影响,探讨其肾脏保护效应。方法:试验设空白对照组(N组)、模型组(DN组)、格列齐特组(GT组)、当归补血汤高/低剂量组(DH/DL组),STZ+高脂饲料喂养诱导糖尿病肾病模型,灌胃8周后取材,检测血糖、血脂及肾功能相关指标,酶联免疫吸附试验检测尿MAU含量,光镜、电镜观察肾脏结构改变。结果:DH组血糖、血脂显著降低,与GT组相当,而HDL高于GT组(P<0.01),肾功能改善,镜下肾脏损伤明显减轻;当归补血汤治疗组MAU低于GT组。结论:当归补血汤可有效改善DM大鼠代谢紊乱,升高血HDL,降低蛋白尿,改善肾功能,减轻肾脏损伤。

Objective： To study the kidney protective effect of Astragals and Angelica Mixture with the influence on serum high density lipoprotein（ HDL） and urinary microalbuminuria（ MAU） in diabetic rats. Methods： STZ diabetic nephropathy model were induced with STZ ＋ high- fat feed,and were randomized into five groups： blank group（ N）,model group（ DN）,gliclazide group（ GT）,high and low Astragals and Angelica Mixture group（ DH/DL）,sacrificed after eight weeks. Measure the blood glucose,lipid,HDL and indicators that related with renal function,enzyme- linked immunosorbent assay to detect the contents of urinary MAU,optical and electron microscope were used to observe the kidney structure change. Results： The DH group had a significantly lower level of GLU、TG and TC that comparable with GT group while the HDL is higher（ P 0. 01）. The Scr and MAU in DH group were declined and microscopically kidney damage was significantly alleviated. Conclusion： Astragals and Angelica Mixture can effectively improve the metabolic disorders of DM rat,elevate blood HDL,reduce proteinuria,improving renal function,alleviates the kidney damage.