Apigenin对黄嘌呤氧化酶活性抑制的实验研究

Study on Inhibitory Effect of Apigenin on Xanthine Oxidase Activity

目的:通过体外酶学试验研究Apigenin对黄嘌呤氧化酶活性的影响及抑制机制。方法:运用紫外分光光度计测定尿酸生成导致的吸光度变化值,测定波长为292 nm,以黄嘌呤为底物根据测定结果绘制Lineweaver-Burk和Dixon图。结果:Apigenin对黄嘌呤氧化酶具有较强的抑制作用,其Ki值为0.151 3μg/ml,抑制类型为混合型;而别嘌呤醇作为参照,其Ki值为0.62μg/ml,抑制类型为竞争性抑制。结论:Apigenin可明显抑制黄嘌呤氧化酶活性。

Objective： To research the influence and inhibitory mechanism of Apigenin on the activity of Xanthine Oxidase by vitro enzymatic test. Methods： Determined the absorbance changes caused by the formation of uric acid value by using UV spectrophotometer,the detection wavelength was 292 nm, according to the measured results, draw the Lineweaver Burk and Dixon plots with Xanthine as substrate. Results： Apigenin had strong inhibition on Xanthine Oxidase, the Ki values was 0.151 3 μg/ml, the type of inhibition is of mixed type; and with Allopurinol as a reference, the Ki value was 0.62 μg/ml, the type of inhibition was competitive inhibition. Conclusion：Apigenin can inhibit the activity of Xanthine Oxidase.