丹参对非酒精性脂肪性肝炎大鼠肝组织NF-κB、TNF-α表达的影响

Effect of Salvia injection on expression of NF-κB、TNF-α in rats liver with non-alcoholic steato-hepatitis

[目的]研究肝组织NF-κB、TNF-α在非酒精性脂肪性肝炎(NASH)中的作用,并探讨丹参注射液对NASH大鼠的保护机制。[方法]采用高脂饮食16周建立大鼠NASH模型,每日予丹参注射液5ml/kg进行干预治疗,免疫组织化学法检测大鼠肝组织TNF-α表达。电泳迁移率改变分析检测大鼠肝组织NF-κB活性,观察丹参对NASH大鼠肝组织NF-κB、TNF-α表达的影响。[结果]与正常组相比,模型组大鼠肝组织NF-κB、TNF-α表达明显升高,丹参治疗后大鼠肝组织NF-κB、TNF-α较模型组明显降低。[结论]肝组织NF-κB、TNF-α表达增强与NASH的发生密切相关,丹参注射液能抑制NASH大鼠肝组织NF-κB、TNF-α表达,达到治疗NASH的目的。

[Objective]To investigate the effect and possible mechanism of Salvia injection on NF-κB and TNF-αin rats liver with non-alcoholicsteato-hepatitis.[Methods]Non-alcoholic steato-hepatitis SD rats model were established by administering a high-fat diet for 16 weeks and were treated with Salvia injection at dosages of 5ml/kg body weight.Immunohistochemistry was used to detect the levels of TNF-αand electrophoretic mobility shift analysis was used to detect the levels of NF-κB in liver tissues.[Results]At the end of 16 th week,the level of NF-κB、TNF-αincreased in model group compared with normal control group,while decreased after treatment with Salvia injection.the difference was statistically significant.[Conclusion]The effect of Salvia injection on preventing Non-alcoholic steato-hepatitis rats could be related to the decrease of the expression of NF-κB、TNF-αin liver tissue.