Rab23通过上调Rac1的水平促进Sa3鳞状细胞癌细胞的侵袭

Rab23 enhances invasion of Sa3 cutaneous squamous cell carcinoma cells via up-regulating the expression of Rac1

目的探讨Ras相关蛋白23(Rab23)对Sa3鳞状细胞癌细胞侵袭能力的影响,以及Rab23是否通过调控Ras相关C3肉毒杆菌毒素底物1(Rac1)促进Sa3细胞的侵袭。方法用慢病毒感染的方式建立Rab23过表达和干涉的Sa3稳定感染细胞系。TranswellTM小室侵袭实验检测Rab23对Sa3细胞侵袭能力的影响;Western blot法检测Rab23对Rac1表达的影响;在稳定感染细胞系中加入Rac1抑制剂ⅡZ62954982后,进行TranswellTM小室侵袭实验检测Rac1在Rab23促进Sa3细胞侵袭中的作用。结果过表达Rab23可以显著增强Sa3细胞的侵袭能力,而干涉Rab23则显著降低Sa3细胞侵袭能力。Rab23促进Rac1的表达。Rac1抑制剂显著抑制Rab23对Sa3细胞侵袭的促进作用。结论 Rab23通过上调Rac1的表达促进Sa3细胞的侵袭。

Objective To investigate the effect of Ras-related protein 23（ Rab23） on the invasion capacity of Sa3 cutaneous squamous cell carcinaoma（ c SCC） cells and determine whether Rab23 can enhance the invasion of Sa3 cells through regulation of Ras-related C3 botulinum toxin substrate 1（ Rac1）. Methods Sa3 cells stably expressing exogenous Rab23 or Sa3 cells deprived of endogenous Rab23 were generated using lentivirus transfection. TranswellTMinvasion assay was used to evaluate the invasion capacity in the above-mentioned cells. The effects of Rab23 overexpression or knockdown on Rac1 expression were assessed using Western blotting. In the last experimental setting,the engineered cells were pretreated with Rac1 inhibitor Z62954982 before being subjected to the assessment of invasion capacity by TranswellTMassay. Results Rab23 overexpression enhanced the invasion ability,whereas ablation of Rab23 attenuated the invasion in Sa3 cells. Rab23 could up-regulate the expression level of Rac1. Finally,using Rac1 inhibitor,we successfully suppressed the invasion capacity of Sa3 cells stably overexpressing Rab23. Conclusion Rab23 may enhance c SCC cell invasion via up-regulating Rac1 signaling.