摘要
目的检测类风湿性关节炎(RA)及骨关节炎(OA)外周血单个核细胞(PBMC)和滑膜成纤维细胞(FLS)白细胞介素27(IL-27)及其受体的水平。方法收集20例RA、20例OA、20例正常人PBMC和4例RA及4例OA患者滑膜组织,培养FLS,采用实时荧光定量PCR法检测PBMC和FLS中IL-27 mRNA的水平;采用免疫细胞化学法检测RA、OA患者FLS IL-27Rα的表达。结果 RA及OA患者PBMC中IL-27 mRNA水平分别是正常人的1.81倍及2.07倍;RA患者和OA患者PBMC中IL-27mRNA水平无明显差异,但RA患者FLS中IL-27 mRNA是OA患者的3.74倍;RA组FLS的IL-27Rα较OA组水平增高。结论 RA、OA患者IL-27水平增加,且RA患者FLS中IL-27水平高于PBMC。
Objective To detect the levels of interleukin 27( IL-27) and its receptor in peripheral blood mononuclear cells( PBMCs) and fibroblasts like synoviocytes( FLS) of patients with rheumatoid arthritis( RA) or osteoarthritis( OA). Methods PBMCs were collected from 20 patients with RA,20 patients with OA and 20 healthy controls. FLS were cultured from synovial tissues of 4 patients with RA and 4 patients with OA. The levels of IL-27 mRNA in PBMCs and FLS were measured using real-time quantitative PCR. The expression of IL-27Rα in FLS was detected using immunocytochemical method. Results The level of IL-27 mRNA in PBMCs of patients with RA or OA was 1. 81 or 2. 07 times of the level in the normal subjects,respectively.There was no significant difference in the IL-27 mRNA level between RA and OA. However,IL-27 mRNA level in FLS of the patients with RA was 3. 74 times of the level in the patients with OA. IL-27Rα expression in FLS of RA group was higher than that in OA group. Conclusion The level of IL-27 in patients with RA and OA increased,and the level of IL-27 in FLS was higher than that in PBMCs in RA patients.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2015年第12期1673-1676,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
湖北省教育厅课题(Q20111909)
湖北省医学领军人才培养工程专项经费(2014年度)