BCL6共抑制因子样蛋白1(BCORL1)在非小细胞肺癌中的表达及意义

Expression and clinical significance of BCL6 corepressor-like 1 in non-small cell lung cancer

目的观察BCL6共抑制因子样蛋白1(BCORL1)在非小细胞肺癌(NSCLC)组织中的表达,并通过下调肺癌A549细胞中BCORL1的水平观察对A549细胞侵袭和迁移能力的影响。方法收集68例NSCLC组织及对应的癌旁组织,应用免疫组织化学染色分析BCORL1和E钙黏素(E-cadherin)在NSCLC及对应癌旁组织中的表达;采用小干涉RNA(siRNA)下调肺癌A549细胞中BCORL1的水平,TranswellTM小室法检测A549肺癌细胞迁移和侵袭能力。结果 BCORL1蛋白在NSCLC组织中表达水平显著高于对应癌旁组织,而E-cadherin蛋白在NSCLC组织中表达水平则显著低于对应癌旁组织;相关性分析证实BCORL1蛋白与E-cadherin蛋白在NSCLC组织中的表达呈显著负相关;临床相关分析发现BCORL1蛋白表达与淋巴结转移和TNM分期具有显著的相关性;特异性siRNA下调BCORL1水平后,E-cadherin蛋白上调;敲低BCORL1后,明显抑制A549肺癌细胞侵袭和迁移。结论 BCORL1在NSCLC组织中高表达且与E-cadherin表达呈显著负相关,其高表达与NSCLC恶性临床病理特征相关。敲低BCORL1,上调E-cadherin表达并抑制肺癌细胞侵袭和迁移能力。

Objective To detect the expression of BCL6 corepressor-like 1（ BCORL1） in tumor tissues of human non-smal cel lung cancer（ NSCLC） and determine the effect of BCORL1 on cell migration and invasion in A549 cells by knockdown of BCORL1. Methods Sixty-eight pairs of NSCLC and nontumor tissues were collected and the expressions of BCORL1 and E-cadherin in them were detected using immunohistochemical staining. The expression of BCORL1 was knocked down by siRNA in A549 cells. TranswellTMassays were performed to test NSCLC cell migration and invasion in vitro. Results The expression of BCORL1 in NSCLC was significantly higher than that in paired noncancerous tissues,while E-cadherin was down-regulated in NSCLC as compared with nontumor tissues. Pearson correlation coefficient analysis suggested that BCORL1 was negatively correlated with E-cadherin expression in NSCLC tissues. Clinical association analysis suggested that the elevated expression of BCORL1 was evidently associated with the higher incidence of lymph node metastasis and more advanced TNM stage. When the expression of BCORL1 was down-regulated by a specific siRNA,E-cadherin was up-regulated,and BCORL1 knockdown obviously inhibited cell migration and invasion in A549 cells. Conclusion BCORL1 is overexpressed in NSCLC tissues and it is negatively correlated with E-cadherin expression. Its high expression is correlated with poor prognostic features. BCORL1 knockdown up-regulates E-cadherin expression and subsequently inhibits cell migration and invasion of lung cancer cells.