摘要
目的检测十堰地区不同Hoehn-Yahr分级的散发性帕金森病患者血浆a-共核蛋白水平的差异。方法选择2012年9月-2015年1月在湖北医药学院附属太和医院就诊的48例原发性帕金森病患者,参照Hoehn-Yahr分级标准分组,对照组选择年龄、性别均与病例组匹配的健康人。采用ELISA方法检测比较48例帕金森病患者与48例正常对照组以及不同Hoehn-Yahr分级PD患者血浆中a-synuclein蛋白寡聚体水平的差异。结果十堰地区48例散发性帕金森病患者血浆中a-synuclein蛋白单体水平与对照组相比无显著差异,PD组寡聚体水平(3.869±1.292 ng/m L)与对照组(2.922±0.804 ng/m L)比较有显著差异,且不同Hoehn-Yahr分级的PD患者血浆a-synuclein蛋白寡聚体水平比较有显著性差异(P<0.05)。结论在十堰市我们首次开展了散发性帕金森病患者血浆a-synuclein寡聚体水平高于正常人,且随着疾病严重及分期的进展,其水平呈上升趋势。因此,血浆a-synuclein蛋白寡聚体可作为帕金森病诊断的有潜力的生物标志物,为协助帕金森病诊断及疾病分期提供帮助。
Objective To explore the plasma α-synuclein concentration in different Hoehn-Yahr grade of sporadic Parkinson＇s disease patients in Shiyan,further to determine the relationship between plasm α-synuclein protein and grade of Parkinson＇s disease. Methods 48 cases of idiopathic Parkinson＇s patients which were from Hubei Medical College Affiliated Taihe Hospital from September 2012 to January 2015 were grouped as Hoehn-Yahr classification standard reference group. The control group were matched with healthy people. ELISA was used to detect α-synuclein protein oligomers concentration. Results α-synuclein protein monomer concentration was compared between the control group and PD group. α-synuclein protein monomer concentration of PD group is（ 3. 869 ± 1. 292 ng / m L）,as well（ 2. 922 ± 0. 804 ng / m L） of control group. There was significant difference（ P〈0. 05） between the two groups. Conclusions Parkinson ＇s disease was found has the higher plasma concentration of α-synuclein oligomers than normal. As the disease progress serious and staging,its concentration rises. Therefore,plasma α-synuclein protein oligomers can be used as diagnosis of Parkinson＇s disease has the potential biomarkers for the diagnosis of Parkinson＇s disease and help to distinguish the disease stage.
出处
《卒中与神经疾病》
2015年第6期323-326,共4页
Stroke and Nervous Diseases
基金
湖北省教育厅科学研究计划指导项目(B2015473)
