HPLC法测定重楼种植药材中4种重楼皂苷的含量

Determination of Four Steroidal Saponins in Cultivated Medicinal Herb Paris Ployphylla by HPLC

目的:建立HPLC法测定重楼中4种甾体皂苷的含量。方法:采用色谱柱为Thermo BDS Hypersil C_(18)(250 mm×4.6 mm,5μm),流动相为乙腈-水梯度洗脱,流速0.8 ml·min^(-1),检测波长为205 nm,柱温为25℃。结果:4种重楼皂苷的标准曲线线性良好(r>0.999 5,n=5),重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ的平均回收率为100.2%,99.93%,97.61%,99.33%;RSD分别为1.29%,1.21%,2.13%,1.84%(n=6);不同批次重楼中4种重楼皂苷的含量有一定差异。结论:该方法准确、简单、快捷,可用于重楼中4种皂苷的定量分析。

Objective： To establish a simultaneous determination method for four steroidal saponins in Paris ployphyUa by HPLC. Methods： The column was Thermo BDS Hypersil C18 （250 mm ×4.6 mm,5 μm）, the mobile phase was acetonitrile-water with gradient elution at a flow rate of 0.8 ml · min-1. The detection wavelength was 205 nm. The column temperature was 25℃. Results： The calibration curves of four steroidal saponins showed good linear relationship （r 〉 0.999 5 ）. The recovery of steroidal saponin Ⅰ,Ⅱ, Ⅵ and Ⅶ was 100.2%, 99.93%, 97.61% and 99.33%, respectively. The RSD was 1.29%, 1.21%, 2.13% and 1.84% （n =6）, respectively. The results showed that the contents of the four steroidal saponins in Paris ployphylla were various among different batches. Conclusion： The method is accurate and convenient, and suitable for the quantitative analysis on four steroidal saponins in Paris ployphylla.