LdNPV对CO2胁迫下舞毒蛾生长发育及生化酶活性影响

Effects of LdNPV on growth, development and biochemical enzymatic activities of Lymantria dispar under CO2 concentration stress

【目的】以森林害虫舞毒蛾为对象,研究CO2介导下LdNPV病毒对舞毒蛾生长发育、解毒酶和保护酶的影响。【方法】利用密闭式CO2人工气候箱研究了不同CO2含量(397、550和750μL/L)条件下饲养的舞毒蛾幼虫,LdNPV对其生长发育指标的影响,并采用分光光度计法测定体内保护酶和解毒酶活性。【结果】高CO2含量胁迫下舞毒蛾3龄幼虫体重累计增长率降低。LdNPV胁迫导致不同CO2含量(397、550和750μL/L)条件下饲养的舞毒蛾3龄幼虫体重累计增长率分别比对照组增加81.27%、71.63%和68.41%;随着CO2含量升高幼虫感染LdNPV死亡率增加,750μL/L高含量处理组死亡率为27.09%。高CO2含量胁迫下舞毒蛾3龄幼虫体内解毒酶CarE和AChE活性随着CO2含量升高而诱导增加,ALP活性随CO2含量增加抑制下降;保护酶CAT活性随着CO2含量升高诱导增加,而SOD活性随着CO2含量升高抑制减少。高CO2含量条件下生长的舞毒蛾3龄幼虫接种LdNPV后,随着CO2含量升高体内CarE、ALP、AChE和CAT活性被抑制,而SOD活性表现为诱导增加。【结论】不同CO2含量下舞毒蛾饲养至3龄幼虫接种LdNPV,随着CO2含量增加,幼虫体重累计增长率减少、死亡率增加。舞毒蛾幼虫CarE、AChE、ALP和CAT活性随着LdNPV处理时间和CO2含量增加而主要表现为抑制减少;SOD活性则主要表现为诱导增加。CO2含量升高可能通过影响生长发育和生理生化增加LdNPV对舞毒蛾幼虫的致病力。

[Objective]In this study,the forest pest Lymantria dispar was used to study the effects of LdNPV on the development,detoxifying enzymes and protective enzymes of Lymantria dispar larvae under CO2 concentration stress.[Method]The effects of LdNPV on the growth and development of L.dispar larvae under different CO2 concentrations(397,550 and 750μL/L)reared into the closed CO2 artificial climate chamber,and in vivo detoxifying enzymes and protective enzyme activities of L.dispar larvae were also determined by spectrophotometry.[Result]The cumulative growth rate of fresh mass of the 3 rd instar larvae of L.dispar decreased under high CO2 concentration stress.The cumulative increase rates of fresh mass of the 3 rd instar L.dispar larvae infected by LdNPV were higher than the control by 81.27%,71.63%and 68.41%,respectively.The mortality rate increased with increasing CO2 concentration,and the cumulative mortality rate of 750μL/L CO2 treatment was 27.09%.Under the high CO2 concentration stress,the activities of detoxifying enzymes CarE and AChE in the 3 rd instar larvae of L.dispar were induced with the increase of CO2 concentration,and the ALP activity was inhibited with the increase of CO2 concentration;the activity of protective enzyme CAT was induced with the increase of CO2 concentration while SOD activity was inhibited with increasing CO2 concentration.However,the activities of CarE,ALP,AChE and CAT in 3 rd instar larvae of L.dispar infected by LdNPV were inhibited with the increase of CO2 concentration,and the activity of SOD was induced with the increase of CO2 concentration.[Conclusion]The duration and cumulative mortality rate of L.dispar larvae infected by LdNPV increased while cumulative growth rate decreased after different CO2 concentraion treatments.The activities of CarE,AChE,ALP and CAT in 3 rd instar larvae of L.dispar decreased with the increase of both the treatment time and CO2 concentration,and the activities of SOD were increased.Therefore,elevated concentration of CO2 may increase the virulence of LdNPV to the L.dispar larvae by affecting the growth,development and biochemical enzymes.