乌骨藤提取物对血液肿瘤细胞的抑制效果及机制研究

Inhibitory effect and mechanism of Marsdenia tenacissima extract on hematological tumor cells

目的探讨乌骨藤提取物抗血液肿瘤的效应及机制。方法体外培养THP-1、MV4-11和HL-60细胞系,用乌骨藤提取物对细胞系进行干预,采用MTS法检测细胞增殖,流式细胞仪检测细胞凋亡,Western blot法分析相关蛋白表达。结果乌骨藤提取物对THP-1、MV4-11、HL-60细胞均有不同程度的抑制生长效果,其中对THP-1、HL-60细胞抑制生长效应最佳,IC50分别为3. 5、3. 02 mg/m L。乌骨藤提取物有明显的促进THP-1、HL-60细胞凋亡效应。乌骨藤提取物作用48 h,凋亡相关因子PARP均出现了降解分裂的条带,THP-1出现了caspase 8条带轻度减弱;而HL-60细胞出现了p-Akt明显减弱。结论乌骨藤提取物可能通过激活与凋亡过程密切相关的蛋白caspase 8及PARP表达,降低p-Akt表达水平,而起到抑制肿瘤细胞增殖、诱导凋亡的作用。

Objective To evaluate the mechanism of anti-hematological tumor of Marsdenia tenacissima extract(MTE),and provide evidence for the treatment of hematological tumor with Marsdenia tenacissima and its preparations.Methods THP-1,MV4-11 and HL-60 were target cells,and Marsdenia tenacissima extract was intervention drugs,and through cell culture in vitro,cell proliferation were determined by MTS assay.Flow cytometry was used detect the cell apoptosis and western blot to analyze the related protein expressions.Results MTE could inhibit cell growth of THP-1,HL-60 and MV4-11,especially THP-1,HL-60 cell lines,IC50 were3.5 mg/m L,3.02 mg/m L respectively.It could also induce apoptosis in THP-1,HL-60 cell lines.After treatment of 48 h,MTE induced cleavage of PARP in HL-60 and THP-1;caspase-8 in THP-1,and down-regulated of p-Akt in HL-60.Conclusion MTE has a significant anticancer activity in AML cell lines,which could suppress the growth and induce apoptosis in AML cells by activating the expression of caspase 8 and PARP,down-regulating p-Akt.