两种方法保存人体透明软骨行异种移植的实验研究

A study on heterotransplant between two kinds of methods in conserving hyaline cartilage.

目的 探讨不同方法保存的人体透明软骨 ,异种移植后软骨存活的动态变化及在整形外科应用的可行性。 方法 选择 4 8只新西兰种成年白兔 ,随机分为两组 ,每组 2 4只 ,分别接受 - 198℃液氮冷冻和 75 %酒精灭活法保存的人体透明软骨移植 ,观察一年。术后每 3个月比较两组血清IgG、IgM、IgA、C3、C4 、PFB(补体B因子 )含量 ,每月随机抽取两组各两只兔子取移植软骨块观察其外形、色泽 ,测重量。比较光学显微镜和透射电镜下的组织学变化。 结果 两实验组各时间点IgG、IgM、IgA、C3、C4 、PFB含量与术前相比无显著性差异 ,P >0 0 5。深低温保存法软骨移植后 1- 2个月内重量略有增加 ,3个月重量开始减少 ,至 1年达最低点。光学显微镜下观察 ,2个月可见新生不成熟软骨细胞。 3个月时软骨开始出现退行性变 ,至 6个月时明显加重。酒精灭活移植软骨重量无明显变化 ,仅在 6个月时开始出现轻度退行性变。透射电镜下观察的变化规律基本与光学显微镜下的观察结果相一致。结论酒精灭活法保存的人体透明软骨用于异种移植后 ,外型变化小 ,提示其临床应用可行性优于液氮冷冻保存法。

Objective To compare the effects of heterotransplant between two kinds of methods in conserving hyaline cartilage. Methods 48 adult white rabbit of New Zealand species were divided into two groups randomly.Each groups received human hyaline cartilage preserved by methods of -198℃ liguid nitrogon cryopreservtion or 75% alcohol inactivation. Serum IgG,IgM,IgA, C 3?C 4? PFB levels were compared each 3 months with that before operation.The contour,color and luster,change of histology of hyaline cartilage were examined in light microscope and electron microscope and the gravity were weight each month. Results IgG,IgM,IgA,C 3?C 4?PFB had no significant change after transplant( P >0 05).The weights of transplanted hyaline cartilage began to increase in 1～2 months and then decreased after 3 months in methods of liguid nitrogon cryopreservation and reached their minimum gravity at the end of one year.Neoformative immature hyaline cartilage were observed after 2 months,slight degeneration were observed after 3 months and degeneration reached their zenith in light microscope after 6 months.The weights had no change in methods of alcohol inactivation and slight degeneration began to occur after 6 months and which were similar in electron microscope. Conclusions Human hyaline cartilage preserved by methods of alcohol inactivation had slight change in contour and had no rejection when they were heterotransplanted.So it suggested that their clinical application feasibility may be better than that of liguid nitrogon cryopreservation.