不同干燥工艺制备的金翘热毒清制剂的抗炎作用

Anti-inflammatory Action of Jinqiao Reduqing Preparation Prepared by Different Drying Process

目的:研究不同干燥工艺制备的金翘热毒清颗粒和金翘热毒清喷雾干燥粉的抗炎作用。方法:将84只小鼠随机分为模型组,阿司匹林组(0.2 g·kg-1),热毒平颗粒组(21.84 g·kg-1),金翘热毒清颗粒组(11.7 g·kg-1),金翘热毒清喷雾干燥粉组(5.85,11.7,23.4 g·kg-1),每组12只,用二甲苯致炎,测定小鼠耳廓肿胀度;另取84只小鼠按上述方法分组和给药,腹腔注射冰乙酸致炎,于590 nm处测定腹腔液吸光度。评价不同干燥工艺制备的金翘热毒清制剂的抗炎作用。结果:模型组,金翘热毒清喷雾干燥粉高、中、低剂量组,金翘热毒清颗粒组,热毒平颗粒组及阳性组小鼠的肿胀度分别为(18.5±3.2),(13.4±2.2),(13.9±3.2),(14.8±3.6),(14.8±2.3),(14.6±4.2),(9.3±2.0)mg,与模型组相比,各给药组动物耳肿胀度均明显降低。模型组,金翘热毒清喷雾干燥粉高、中、低剂量组,金翘热毒清颗粒组,热毒平颗粒组及阳性组的A分别为0.677±0.121,0.512±0.099,0.523±0.137,0.583±0.195,0.537±0.065,0.558±0.107,0.321±0.094。结论:金翘热毒清颗粒和金翘热毒清喷雾干燥粉均具有一定的抗炎作用,可抑制鼠耳肿胀和腹腔毛细血管通透性增高;等剂量金翘热毒清喷雾干燥粉的抗炎作用稍强于金翘热毒清颗粒,等临床倍数剂量的热毒平颗粒的抗炎作用稍弱于金翘热毒清喷雾干燥粉。

Objective： To investigate anti-inflammatory effect of Jinqiao Reduqing granules and spray drying powder with different drying process. Method： Eighty-four mice were randomly divided into model group,aspirin group（ 0. 2 g·kg^-1）,Reduping group（ 21. 84 g·kg^-1）,Jinqiao Reduqing granules group（ 11. 7 g·kg^-1）and Jinqiao Reduqing spray drying powder group（ 5. 85,11. 7,23. 4 g·kg^-1）,twelve for each group; then with xylene-induced inflammation,mouse ear swelling was measured. In addition,an alternative 84 mice were divided into groups and treated with the same method; inflammation was induced by traperitoneal injection of acetic acid,and absorbance of peritoneal fluid was measured at 590 nm. Anti-inflammtory effect was evaluated by reducing xylene-induced ear swelling level and glacial acetic acid-induced abdominal capillary permeability. Result： Mouse ear swelling of each group were（ 18. 5 ± 3. 2）,（ 9. 3 ± 2. 0）,（ 14. 6 ± 4. 2）,（ 14. 8 ± 2. 3）,（ 14. 8 ± 3. 6）,（ 13. 9 ± 3. 2）,（ 13. 4 ± 2. 2） mg,respectively. Compared with the model group,swelling and swelling rate of each drug administration groups had significantly decreased. Absorbance values of each group were 0. 677 ±0. 121,0. 321 ± 0. 094,0. 558 ± 0. 107,0. 537 ± 0. 065,0. 583 ± 0. 195,0. 523 ± 0. 137 and 0. 512 ± 0. 099,respectively. Conclusion： Jinqiao Reduqing spray drying powder and granules has a certain anti-inflammatory effect,they can obviously decrease mouse ear swelling and capillary permeability in abdominalcavity. But at the same dose,anti-inflammatory effect of Jinqiao Reduqing spray drying powder was more powerful than that of Jinqiao Reduqing granules, and under the same doses of equal clinical multiple, anti-inflammatory effect of Reduping granules was slightly weaker than that of Jinqiao Reduqing spray drying powder.