骨髓间充质干细胞向神经元样细胞分化中黄芪多糖的诱导作用

Effect of astragalus polysaccharides on the neuronal differentiation of bone marrow mesenchymal stem cells

背景:黄芪有效组分的抗氧化作用被认为是诱导骨髓间充质干细胞向神经元样细胞分化的原因。目的:观察黄芪多糖联合碱性成纤维生长因子诱导骨髓间充质干细胞向神经元细胞分化的效果。方法:取第3代人骨髓间充质干细胞用碱性成纤维生长因子进行神经前体细胞诱导分化24 h,随后加入黄芪多糖继续培养1-3 d,同时设空白对照组和单纯碱性成纤维生长因子诱导组。免疫细胞化学及蛋白印迹法检测巢蛋白和神经元特异性烯醇化酶的表达。结果与结论:与单纯碱性成纤维生长因子诱导组相比,碱性成纤维生长因子+黄芪多糖诱导组的神经元特异性烯醇化酶阳性细胞比例较高(P<0.05);单纯系统碱性成纤维生长因子诱导组和碱性成纤维生长因子+黄芪多糖诱导组皆有巢蛋白条带表达,利用凝胶成像系统分析,碱性成纤维生长因子+黄芪多糖诱导组的灰度值大于单纯碱性成纤维生长因子诱导组(P<0.05)。结果可见黄芪多糖联合碱性成纤维生长因子诱导骨髓间充质干细胞向神经元样细胞分化具有更高的诱导分化效率。

BACKGROUND： Active components of Astragalus have an antioxidant effect, which is considered to result in the neuron-like differentiation of bone marrow mesenchymal stem cells.OBJECTIVE： To investigate the effect of astragalus polysaccharides combined with basic fibroblast growth factor to induce the differentiation of bone marrow mesenchymal stem cells into nerve cells. METHODS： After 24 hours of pretreatment with basic fibroblast growth factor, passage 3 human bone marrow mesenchymal stem cells were cultured with astragalus polysaccharides for 1-3 days（combined group）. Blank control group and basic fibroblast growth factor group were set up. Expression of neuron-specific enolase and nestin was detected using western blot or immunocytochemical staining. RESULTS AND CONCLUSION： The expression of neuron-specific enolase was higher in the combined group than the basic fibroblast growth factor group（P〈0.05）. Expression of nestin was found in both basic fibroblast growth factor group and combined group, but the gray value was higher in the combined group than the basic fibroblast growth factor group（P〈0.05）. These findings indicate that astragalus polysaccharides combined with basic fibroblast growth factor is better to induce the neuronal differentiation of bone marrow mesenchymal stem cells.