摘要
为了建立起一种同时具备快速、特殊、敏感以及有效的对霍乱弧菌进行检测的办法,并且借此来控制或预防该细菌造成大面积感染的可能性,对这种实际存在的霍乱弧菌中存在的ctx A基因所拥有的保守区域进行引物设计,通过实时浊度仪来进行LAMP检测,即环介导等温扩增研究法,对其所具备的特性以及敏感性进行研究分析,并且通过这一方法对极高可能存在海产品中的霍乱弧菌进行检测。
In order to create a rapid, specific, sensitive and effective detection of vibrio cholera, and thereby to control or prevent extensive infection caused by the bacteria, the conservative area of ctx A gene that exist in the vibrio cholerae have were primer designed, and LAMP detection was done by real-time turbidity instrument, named ring mediated isothermal amplification method. Its characteristics and sensitivity analysis were studied, and through the method the vibrio cholera with high existence in seafood was detected.
出处
《食品工程》
2015年第4期25-28,共4页
Food Engineering
