环氧化酶抑制剂对急性不完全脊髓损伤核转录因子κB及CD4^+/CD8^+的影响

Effects of cyclooxygenase inhibitors on nuclear factor κB and CD4^+/CD8^+ expression in rat models of acute incomplete spinal cord injury

背景:塞来昔布治疗急性大鼠脊髓损伤,较目前通用的甲强龙具有不良反应少、价格低廉等优点,但是剂量尚无法准确把握。目的:探讨不同剂量环氧化酶抑制剂对急性不完全脊髓损伤核转录因子κB及CD4^+/CD8^+的影响。方法:采用改良Allen方法建立大鼠T_8及T_9段脊髓不完全损伤模型。将60只造模成功的大鼠随机等分为4组:单纯损伤组,不使用任何药物;塞来昔布小、中、大剂量组,分别于脊髓损伤30 min后灌胃塞来昔布25,50,100 mg/kg。结果与结论:塞来昔布治疗后,脊髓损伤大鼠神经功能明显改善,脊髓中核转录因子κB表达水平明显降低,外周血中CD4^+和CD8^+T细胞数量明显减少,且小剂量的效果更佳。提示不同剂量的塞来昔布均可治疗急性创伤性脊髓损伤,且小剂量(25 mg/kg)的效果更佳。

BACKGROUND： Celecoxib has advantages including fewer adverse reactions and low cost over the commonly used methylprednisolone in the treatment of acute spinal cord injury, but the effective dose cannot be well controlled.OBJECTIVE： To investigate different doses of cyclooxygenase inhibitors on nuclear factor κB and CD4^＋/CD8^＋ expression in rat models of acute incomplete spinal cord injury. METHODS： Rat models of incomplete spinal cord injury in T8 and T9 segments were established using modified Allen method. Sixty successful modeling rats were randomly and evenly divided into 4 groups： simple injury group and low, medium-and high-dose of celecoxib groups. Rats in simple injury group were not applied any drugs. Rats in low-, medium- and high-dose celecoxib groups were respectively intragastrically administered 25, 50 and 100 mg/kg celecoxib after 30 minutes of spinal cord injury. RESULTS AND CONCLUSION： After celecoxib treatment, rat neurological function was significantly improved. Nuclear factor κB expression level in spinal cord was significantly decreased. CD4^＋ and CD8^＋ T cell numbers in peripheral blood were significantly decreased. Low dose of celecoxib treatment had a better outcome. These results suggest that different doses of celecoxib can treat acute traumatic spinal cord injury, and low-dose（25 mg/kg） celecoxib treatment can achieve a better outcome.