雌二醇和孕酮对去势模型小鼠子宫内膜巨噬细胞及相关因子表达的影响

Effect of estradiol and progesterone on the number of macrophages and the expression of related factor in the endometrium of ovariectomized mice

背景:有研究表明,巨噬细胞功能及巨噬细胞集落刺激因子的表达可能受雌、孕激素直接或间接调控,但具体影响尚未深入研究。目的:观察雌二醇和孕酮对双侧卵巢切除小鼠子宫内膜巨噬细胞及巨噬细胞集落刺激因子表达的影响。方法:将双侧卵巢切除的小鼠随机分为雌激素组、孕激素组和对照组,分析各组小鼠子宫内膜巨噬细胞的数量和巨噬细胞集落刺激因子的表达强度。结果与结论:免疫组织化学染色结果显示,与对照组相比,雌激素组与孕激素组小鼠子宫内膜巨噬细胞数量、干预6,24 h后巨噬细胞集落刺激因子的表达明显高于对照组(P<0.05)。结果证实,雌二醇和孕酮都对子宫内膜巨噬细胞有趋化作用,并可以诱导巨噬细胞集落刺激因子的表达。

BACKGROUND： Studies have shown that macrophage function and macrophage colony-stimulating factor expression may be directly or indirectly affected by regulation of estrogen and progestational hormone, but the specific impact has not been studied in depth.OBJECTIVE： To observe the effect of estradiol and progesterone on the number of macrophages and the expression of macrophage colony-stimulating factor in the endometrium of bilaterally ovariectomized mice. METHODS： The bilaterally ovariectomized mice were randomly divided into estrogen, progestational hormone, and control groups. The number of macrophages and the expression of macrophage colony-stimulating factor in the endometrium of mice in each group were analyzed. RESULTS AND CONCLUSION： Immunohistochemical staining results showed that after 6 and 24 hours of intervention, the number of macrophages and the expression of macrophage colony-stimulating factor in the mouse endometrium in the estrogen and progestational hormone groups were significantly higher than those in the control group（P 〈0.05）. These results confirm that both estradiol and progesterone can increase the number of macrophages, and can increase the expression of macrophage colony-stimulating factors in the endometrium of bilaterally ovariectomized mice.