圆锥绣球种子离体萌发的培养条件

In vitro Seed Germination of Hydrangea paniculata

为克服圆锥绣球种子萌发困难,提高种子萌发率,推动品种繁殖及选育,以贵州施秉野生圆锥绣球为材料,测量不同储藏条件、消毒处理、培养基类型和暗处理时间下种子的萌发及生长指标。结果表明:4℃低温干燥储藏条件最佳,4个月的发芽率和发芽指数仍高达90.9%和7.9;使用75%酒精消毒30s后再用8%次氯酸钠消毒1min可以消除种子污染,且发芽势、发芽指数、活力指数分别达38.8、10.1、103.9;不同培养基对圆锥绣球种子萌发有显著影响,B-5培养基显著高于其他培养基,发芽指数及活力指数分别达10.0和126.7;暗处理0d时,发芽率、发芽势、发芽指数和活力指数最高,分别达90.7%、31.4、8.5和85.0。因此,圆锥绣球种子离体保存及培养条件选择4℃低温干燥保存、利用75%酒精消毒30s后再用8%次氯酸钠消毒1min,经过B-5培养基培养且不需要暗处理。

H.paniculataseeds are hard to germinate,in order to overcome this problem,enhance seed germination rate,promote its breeding and propagation,the authors measured the germination and growth indexes of seeds with various storage conditions,sterilization treatment,media type and dark treatment.Results：The best storage condition is 4℃ with low humility,which can maintain the germination rate and germination indicator to 90.9% and 7.9.Sterilization with 30s75% alcohol and 1min 8% NaClO can eliminate the contamination and the germination energy,germination indicator and vigor index were 38.8,10.1and 103.9respectively.Different media have significant affection with seeds germination.B-5media has the best performance with the highest germination indicator and vigor index,which were 10.0and126.7separately.Seeds without any dark treatment have the best performance,the germination rate,germination energy,germination indicator and vigor index were 90.7%,31.4,8.5and 85.0.So the H.paniculataseeds should be stored in the 4℃dry environment and sterilize with 30s75%alcohol and 1min8%NaClO then propagated in the B-5media without any dark treatment.