解淀粉芽孢杆菌CMN1308对栗实病原菌的诱导抗性

Inducible Resistance of Bacillus amyloliquefaciens CMN1308 against Pathogenic Fungi of Chestnut

为探明CMN1308的作用机制,为其开发应用提供理论依据,采用接种培养和酶活测定的方法,研究其对扩展青霉、腐皮镰刀菌、栗疫菌和黑曲霉等4种主要栗实致病菌的抗生作用;并在此基础上,以过氧化物酶（POD）、多酚氧化酶（PPO）酶活性及丙二醛（MDA）含量的变化作为指标,研究解淀粉芽孢杆菌CMN1308对扩展青霉的诱导抗性。结果表明：解淀粉芽孢杆菌CMN1308原液、菌悬液和滤液对4种栗实致病菌均具有不同程度的防治效果。其中,解淀粉芽孢杆菌CMN1308原液的防治效果最好,4种病菌的发病率仅为28.3%-50%。解淀粉芽孢杆菌CMN1308能够显著增强栗实的POD和PPO酶活性,降低MDA含量,提高栗实对扩展青霉的抵抗力。

The inducible resistance of Bacillus amyloliquefaciens CMN1308 against Penicillium expansum,Fusarium solani,Cryphonectria parasitica and Aspergillus niger of chestnut by inoculated culture and enzymatic determination methods and the inducible resistance of B.amyloliquefaciens CMN1308 against P.expansum of chestnut by analyzing POD and PPO activity and MDA content of chestnut to explore the action mechanism of B.amyloliquefaciens CMN1308 and provide the theoretical basis for development and application of B.amyloliquefaciens CMN1308.Results：The original liquid,suspension and filter liquid of B.amyloliquefaciens CMN1308 have different control effects against four diseases to a varying degree.The original liquid of B.amyloliquefaciens CMN1308 has the best control effect against four diseases and the morbidity of four diseases is 28.3% -50%.B.amyloliquefaciens CMN1308 improves the resistance against P.expansum of chestnut by significantly enhancing POD and PPO activity and reducing MDA content of chestnut.