美国进境黑胡桃木中截获1种昆虫的分子鉴定

Molecular Identification of 1 Insect Species in Juglans nigrafrom USA

为了对口岸截获昆虫标本的快速鉴定提供理论依据,采用DNA条形码技术,对广东南海口岸从美国进境黑胡桃木(Juglans nigra L.)中截获的1种昆虫进行分子鉴定,并与GenBank中相关的序列进行比对。结果表明:截获昆虫样品基因序列与胡桃木细小蠹(Pityophthorus juglandis Blackman 1928)最为接近,结合形态特征,综合判定该虫为胡桃木细小蠹。

In order to provide theoretical basis for rapid identification of insect specimen in ports,The technique of DNA barcoding was applied to identify the inscet intercepted by Nanhai Entry-Exit Inspection and Quarantine Bureau in the imported J.nigrafrom America in January 2015,blasted with released data in GenBank database.The Results show that the intercepted insect sample gene sequence and walnut pityophthorus（Pityophthorus juglandis Blackman 1928）comes losest.According to morphological characters and sequence,the insect was identified as Pityophthorus juglandis（Blackman 1928）.