摘要
建立同时测定靓靓胶囊中没食子酸、葛根素、芍药苷及阿魏酸含量的方法。采用高效液相色谱法,对有效成分进行分离并测定其含量,色谱条件为Waters Symmetry C18(4.6mm×250mm×5μm)液相色谱柱,以乙腈-0.02%三氟乙酸水溶液为流动相,梯度洗脱;检测波长250nm(没食子酸,葛根素,芍药苷);316nm(阿魏酸),流速为1.0m L·min-1,柱温30℃。在上述色谱条件下,没食子酸,葛根素,芍药苷,阿魏酸之间有良好的分离度,没食子酸、葛根素、芍药苷、阿魏酸的线性范围分别为11.54-144.23μg·m L-1(r=1);26.67-160.04μg·m L-1(r=0.9998);12.45-124.45μg·m L-1(r=0.9997);1.00-10.01μg·m L-1(r=0.9996)。4个成分的平均回收率分别为没食子酸100.37%,葛根素98.44%,芍药苷98.89%,阿魏酸98.72%(RSD分别为1.78%,2.14%,1.83%,1.78%)。线性考察,精密度试验,重复性试验,稳定性试验,加样回收率试验均符合方法学验证试验相关要求。本方法简便,准确,重复性好,专属性好,适用于靓靓胶囊的质量控制。
To establish an HPLC method for simultaneous determination the contents of gallic acid, puerarin,paeoniflorin and ferulic acid in Liangliang capsule. The method used high performance liquid chromatography to separate the effective components and determine its content. The analysis was performed on a Waters Symmetry C18column（4.6mm×250mm×5μm）. The mobile phase was composed of acetonitrile-0.02% trifluoroacetic acid with gradient elution at the flow rate of 1.0m L·min^-1. The detection wavelengths were set at 250nm（gallic acid, puerarin,paeoniflorin） and 316 nm（ferulic acid）. The column temperature was 30℃. Gallic acid, puerarin, paeoniflorin and ferulic acid were well separated by this method. The linearities of gallic acid, puerarin, paeoniflorin and ferulic acid were good in the ranges of 11.54-144.23μg·mL^-1（r=1）, 26.67-160.04μg·mL^-1（r=0.9998）, 12.45-124.45μg·mL^-1（r=0.9997）, 1.00-10.01μg·mL^-1（r=0.9996）, respectively. The average recoveries of gallic acid, puerarin, paeoniflorin and ferulic acid were 100.37%, 98.44%, 98.89%, 98.72%, with corresponding RSD were 1.78%, 2.14%,1.83%, 1.78%. The results of linear relation test, precision test, repeatability test, stability test and the sample recovery test satisfied the demand of method verification test. The method is simple, accurate and reproducible, can be used for the quality evaluation of Liangliang capsule.
出处
《食品与发酵科技》
CAS
2015年第6期68-72,共5页
Food and Fermentation Science & Technology
