摘要
目的对比化学发光酶免疫分析法及酶联免疫吸附试验(ELISA)用于丙型肝炎抗体检测的效果。方法选取丙型肝炎患者140例,抽取血液标本后分别进行丙型肝炎病毒(HCV)相关抗体的化学发光酶免疫分析法及ELISA法检测。结果化学发光酶免疫分析法检出抗-HCV阳性的检出率为98.6%,高于ELISA法的检出率(94.3%),差异有统计学意义(P<0.05)。化学发光酶免疫分析法对于抗AMA-M2抗体、抗3E抗体、抗SP100抗体、抗PML抗体、抗GP210抗体的检测阳性率分别为80.0%、73.6%、37.9%、55.7%和47.9%,而ELISA法检测结果分别为12.9%、12.9%、13.6%、10.7%和6.4%,差异均有统计学意义(P<0.05)。结论相对于ELISA法,化学发光酶免疫分析法在丙型肝炎中的应用有较高的检出阳性率,对于相关抗体检测有较高的敏感性,值得推广应用。
Objective To compare the effects of chemiluminescent enzyme immunoassay and ELISA method for the detection of hepatitis C antibodies. Methods 140 inpatients with hepatitis C in our hospital were selected and the blood samples were collected for detecting hepatitis C antibodies by adopting the chemiluminescence immunoassay and ELISA method. Results The positive detection rate of anti hepatitis C virus（HCV） antibody detected by the chemiluminescent enzyme immunoassay was 98.6 %,which was higher than 94.3 % detected by the ELISA method, the difference was statistically significant （P〈0.05）. The positive rates of chemiluminescent enzyme immunoassay for detecting anti AMA-M2 antibody, anti-3 E antibody, anti-SP100 antibody, anti-PML antibody and anti-GP210 antibody were 80.0% ,73.6% ,37.9% ,55.7% and 47.9%. respectively,while which detected by the ELISA method were 12.9 %, 12.9%, 13.6%, 10.7 % and 6.4 % respectively, the differences were statistically significant （P〈0.05）. Conclusion Compa.red with the ELISA method,the chemiluminescent enzyme immunoassay has very good positive detec- tion rate in hepatitis C, has better sensitivity for the relevant antibody detection, at the same time has higher detection sensitivity and is worthy of being promoted and applied.
出处
《检验医学与临床》
CAS
2016年第1期18-20,共3页
Laboratory Medicine and Clinic
基金
广东省医学科研基金立项课题(A2014054)
