应用CantoⅡ流式细胞仪建立14～16岁人群CD64正常参考值的探讨

Establishment of normal reference value for CD64 in healthy adolescents aged 14-16 years by CantoⅡ flow cytometer

目的探讨应用CantoⅡ流式细胞仪建立14~16岁青少年人群的CD64正常参考值。方法选取14~16岁健康体检者52例,静脉抽取外周血,运用CantoⅡ流式细胞仪检测CD64的表达,并进行统计学分析。结果 CD64在单核细胞中表达最高,在淋巴细胞中几乎不表达,在中性粒细胞中平均荧光强度为782.78,95%正常参考值范围为211.78~1 353.78,CD64指数的单侧95%正常参考值范围为〈1.53。结论初步建立了应用CantoⅡ流式细胞仪检测CD64正常参考值的方法,为基础研究及临床诊断提供参考。

Objective To investigate the normal reference value establishment of CD64 in the healthy adolescents aged 14-16 years by the Canto Ⅱ flow cytometer. Methods The peripheral blood samples collected from 52 healthy adolescents aged 14-16 years were detected the CD64 expression by using the BD Canto Ⅱ flow cytometer, and the results were statistically analyzed. Results The expression of CD64 was highest in monocytes,but almost no expression was found in lymphocytes. The CD64 mean fluorescence intensity （MFI） in neutrophils was 782.78, its 95% normal reference range was 211.78-1 353.78,the 95% normal reference range of CD64 index was less than 1.53. Conclusion The method for detecting the normal reference value of CD64 in the healthy adolescents by the CantoⅡ flow eytometer is preliminarily established,providing a reference for the basic researches and clinical dianosis.