疏风止痛方有效成分体外透皮实验研究

Vitro Transdermal Experiment of Effective Components in Shufeng Zhitong Fang

目的:以藁苯内酯、欧前胡素为指标考察疏风止痛方的透皮吸收特性。方法:采用Franz扩散池法,以大鼠腹皮为模型,UPLC法测定指标成分的累积渗透量。结果:选择乙醇/PEG400/生理盐水(1∶1∶8)作为该实验接收液;随着给药量的增加藁苯内酯的累积渗透量变化不大,而欧前胡素的累积渗透量随着给药量的增加有降低的趋势;模型拟合结果显示藁苯内酯和欧前胡素的透皮行为分别符合一级和Higuchi方程。结论:疏风止痛方具有经皮渗透特性性,为其透皮吸收给药途径提供了依据。

Objective：To study the effect of Shufeng Zhitong Fang on transdermal behavior by the dosage administration of ligustilide and imperatorin. Methods：The abdomen skin was used as models to study the transdermal absorption characteristic by Franz diffusion cell method. The sample content was determined by UPLC. Results：The ethanol/PEG400/Normal saline（1∶1∶8）was selected as receive solution in Shufeng Zhitong Fang transdermal experiment. With the increase of the administration dosage,the cumulative penetration of ligustilid had changed little and imperatorin had decreased;the result showed that the ligustilid and Imperatorin transdermal behavior model conform to Higuchi and an equation. Conclusion：Shufeng Zhitong Fang had the penetretion and absorption properties,which made a foundation for its vitro transdermal administration.