HPLC-MS/MS法测定巴天酸模中7个蒽醌类成分含量

Determination of Seven Anthraquinones Constituents in Polygonum Rumex Patientia L. by HPLC-MS/MS

目的:采用HPLC-MS/MS法测定巴天酸模中7个蒽醌类成分的含量。方法:采用SB-C_(18)色谱柱(4.6 mm×100 mm,1.8μm),流动相为乙腈(A)-0.1%甲酸水(B)。梯度洗脱程序为:0~8.5 min,95%~95%A;8.5~8.51 min,95%~40%A;8.51~12 min,40%~40%A。流动相流速为0.4 mL/min,柱温为20℃,进样量为10μL。结果:大黄酸、芦荟大黄素在2.5~50 ng/mL,大黄素、大黄素甲醚在10~250 ng/mL,大黄酚在25~1000 ng/mL,大黄素-8-O-葡萄糖苷、大黄素甲醚-8-O-葡萄糖苷在10~500 ng/mL范围内,进样量与峰面积具有良好线性关系(r^2均>0.98)。同时,其平均加样回收率在90.32%~106.75%(n=6)。结论:该方法简便、准确、灵敏,可用于巴天酸模中蒽醌类成分的含量测定。

Objective：To establish a HPLC-MS/MS method for determining the content of seven anthraquinones constituents in Polygonum Rumex Patientia L.Methods：A SB-C_（18） column（4.6mm×100 mm,1.8μm） was used with a mobile phase of acetonitrile（A）-0.1%acetic acid in water（B）.The gradient elution program was as follows：0~8.5 min,95%~95%A;8.5~8.51 min,95%~40%A;8.51~12 min,40%~40%A.The column temperature was set at 20 °C.The flow rate was kept at 0.4 mL/min and the sample volume was 10μL.Results：All calibration curves had good linearity（r20.98）over the concentration ranges of 2.5-50 ng/mL for rhein and aloe rhein,10-250 ng/mL for emodin and physcion,25~1000 ng/mL for chrysophanol,10~500 ng/mL for emodin-8-O-glucoside and physcion-8-O-glucoside.And the average recoveries were all ranged in 90.32%~106.75%（n=6）.Conclusion：The method is simple,accurate and sensitive for the determination of anthraquinones constituents in Polygonum Rumex Patientia L..