杜仲内生细菌DZBG07对小麦全蚀病的生防作用

Biocontrol effect of endophytic bacteria DZBG07 from Eucommia ulmoides oliver on wheat take-all

以获得的一株对小麦全蚀病菌有较好抑菌活性的杜仲内生细菌DZBG07为试验菌株,研究其对小麦全蚀病的防治效果,分析拮抗细菌DZBG07对不同生育期的小麦植株根部中多酚氧化酶(PPO)、过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)活性的影响。结果表明,杜仲内生细菌DZBG07能增强小麦对全蚀病的抗性,其苗期小麦全蚀病的发病率仅为26.57%,明显低于未经DZBG07处理的小麦全蚀病的发病率((86.35%);且经杜仲内生细菌DZBG07处理的小麦植株,在不同生育期其根部的多酚氧化酶(PPO)、过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)3种防御酶活性均有所提高。杜仲内生细菌DZBG07对小麦全蚀病的抗性研究为揭示其生防机理与应用该菌提供了科学依据。

In the study, the endophytic bacteria DZBG07 showing strong activity against wheat Gaeumannomyces graminis was re-searched for its the control effect to wheat take-all, and the effect of inoculation of DZBG07 in the wheat rhizosphere on related enzyme activity of wheat roots in different growth stages was analysed through pot experiments. The results indicated inoculation of DZBG07 in the wheat rhizosphere reduced significantly the incidence of wheat take-all, the incidence of wheat take-all by DZBG07 was 26. 57%, which was lower than that of the normal control groups（86. 35%）, and the activities of polyphenol oxidase, peroxidase and phenylala-nin ammonia-lyase of wheat roots treated with of DZBG07 were significantly higher than those in normal control groups. The resistance of DZBG07 to wheat take-all afforded scientific basis for its application and the further research on bio-controlling mechanism.