回补维生素E对维生素E缺乏不孕大鼠妊娠结局的影响

The effect of vitamin E supplement on the pregnancy outcomes of vitamin E-deficiency-associated infertile female rat

目的研究在植入过程回补维生素E对维生素E缺乏不孕(VED)大鼠妊娠结局的影响。方法从出生后11d开始喂养维生素E缺乏的食物,喂养6个月后建立VED大鼠模型。在模型大鼠交配后5.5d、6.5d、7.5d分别灌胃500mg/kg的维生素E回补,以非VED大鼠为对照组,在回补0h、4h、8h、12h、16h、20h和24h观察维生素E的浓度变化,并于交配后19.5d统计大鼠子宫的着床点个数、活胎个数和胎鼠体重变化。结果 VED大鼠在回补4h后血浆中维生素E的浓度为13.54±3.81μg/ml,达到正常水平;分别在交配后5.5d、6.5d、7.5d回补维生素E的VED大鼠妊娠率分别为80%、60%、40%(对照组均为100%),着床点个数分别为9.3±1.1、8.5±0.5和8.4±1.4(对照组为12.7±0.5、13.0±2.2和12.7±1.2),活胎个数分别为5.0±2.0、4.0±2.3和2.0±2.4(对照组为12.3±0.5、12.7±2.4和11.3±1.7),均显著低于对照组(P<0.05);交配后5.5d回补组胎鼠体重与对照组相比无统计学差异(P>0.05),交配后6.5d和7.5d回补组胎鼠体重显著低于对照组(分别为1.74±0.55g和1.63±0.68g vs.2.16±0.18g和2.19±0.21g)(P<0.01)。结论在植入过程回补维生素E可部分挽救由于维生素E缺乏所导致的不孕;随着回补时间的延后,回补维生素E对妊娠结局挽救的效果明显下降。

Objectives. To study the effect of vitamin E （VE） supplement during the implantation process on pregnancy outcome of VE deficiency-associated infertile female rat. Methods： Female rats were on VE-deficient diet to establish the VE deficiency-associated infertility model （VED group）. The model rats （VED group） were given VE （500 mg/kg） by intragastric administration on 5.5,6.5 and 7.5 days post-coitum （dpc） respectively,with the non-VED rat （on normal diet） supplemented with vehicle as control. Plasma VE levels were determined at 0 h,4 h,8 h,12 h,16 h, 20 h and 24 h after supplement. The pregnancy outcomes were determined on 19.5 dpc,with number of implantation sites, live fetus and weight of fetus examined. Results： Plasma VE levels in VED group reached the normal levels at 4 h after VE supplement. The pregnancy rates in VED group with VE supplement at 3 diferent time-points （subgroups 5.5,6.5 and 7.5 dpc） were 80%,60% and 400% respectively （100% in control）,the number of implantation sites were 9.3±1.1,8.5±0.5 and 8.4±1.4 respectively （in control,12.7±0.5,13.0±2.2 and 12.7±1.2）,and the number of live fetus were 5.0＋2.0,4.0±2.3 and 2.0±2.4 respectively （in control, 12.3±0.5,12.7± 2.4 and 11.3±1.7）. There was a significant difference in all observed parameters between VED group and control group at all subgroups （P 〈 0. 05）. Compared with that in control group, fetus weight was significantly reduced in VED group with VE supplement at 6.5,7.5 dpc （1. 74±0. 55 g,1. 63±0.68 g vs. 2. 16±0.18 g,2. 19±0.21 g） （P〈0.01） ,while no significant difference was found between VED subgroup of 5.5 dpc supplement and normal control（P〈0.05）. Conclusion： VE supplement during the implantation process can partially rescue the infertility caused by VE deficiency in rat. The efficacy of rescue was getting lower with the time delayed.