银纳米颗粒对水葱早期生长和生理特征的影响

Effects of silver nanoparticle on the early growth and physiological characteristics of Scirpus tabernaemontani

选取挺水植物水葱(Scirpus tabernaemontani)为受试物种,研究柠檬酸钠-银纳米颗粒(N-AgNPs)对水葱早期生长和生理特性的影响.结果表明:N-AgNPs显著促进了水葱叶片的生长,与N-0 mg·L^(-1)(对照组)相比,N-5 mg·L^(-1)、N^(-1)0 mg·L^(-1)、N^(-1)5 mg·L^(-1)、N-20 mg·L^(-1)处理组水葱叶片的生长速率分别增加20.7%、37.6%、42.9%、47.7%,生物量分别增加77.3%、158.2%、95.5%、127%,叶片中叶绿素含量分别增加23.3%、67.7%、85.2%、80.5%.N-AgNPs浓度与水葱的生长速率、生物量、叶绿素的含量呈正相关关系.另一方面,N-AgNPs浓度增大提高了光系统Ⅱ(PSⅡ)反应中心的光能转换效率(F_v/F_m),且N-AgNPs浓度与F_v/F_m呈显著正相关关系.N-5 mg·L^(-1)处理组水葱叶片PSⅡ功能反应中心开放度(ΔFv/Fm)最大,光合电子传递速率(ETR)最快,光合电子传递活性(qP)最大.然而,随着N-AgNPs浓度增大,水葱叶片PSⅡ功能反应中心开放度减小,光合电子传递速率逐渐变慢,光合电子传递活性逐渐降低.另外,N-AgNPs浓度增加降低了水葱叶片中的丙二醛含量,膜脂过氧化作用减弱;N-AgNPs浓度增加降低了水葱叶片中脯氨酸含量,水葱自我调节能力减弱.

The effects of Na-Citrate coated silver nanoparticles（N-AgNPs） on early growth and physiological characteristics of Scirpus tabernaemontani were investigated. N-AgNPs significantly promoted the leaf growth rate ofScirpus tabernaemontani. Compared with the N-0 mg·L-1group（control group）,the leaf growth rates increased by 20.7%, 37.6%, 42.9% and 20.7%, the biomass accumulation increased by 77.3%, 158.2%, 95.5% and 77.3%, and the chlorophyll content in leaves increased by 23.3%, 67.7%, 85.2% and 67.7% in N-5 mg·L-1, N-10 mg·L-1,N-20mg·L-1 group,respectively.Positive correlations were found between N-AgNPs concentration and leaf growth rate, biomass and chlorophyll content. On the other hand,the PSⅡreaction center of light energy conversion efficiency（Fv/F_m） improved with the increase of N-AgNPs concentration, indicating their significant positive correlation. During the treated groups, PSⅡfunction reaction center of N-5 mg·L^（-1）group exhibited a highest opening degree（ΔFv/Fmreached max）, biggest photosynthetic electron transport rate（ ETR reached max）, and a biggest photosynthetic electron transport activity（ qP reached max）.However, with the increase of N-AgNPs concentration, PS Ⅱ function reaction center opening degree, photosynthetic electron transport rate and photosynthetic electron transport activity gradually decreased. In addition, the malondialdehyde content in leaves decreased with the increase of N-AgNPs concentration, which revealed that N-AgNPs could reduce the membrane lipid peroxidation ofscirpus tabernaemontani. The proline content in leaves decreased with the increase of N-AgNPs concentration, which suggested that N-AgNPs could reduce the self-regulation ability ofscirpus tabernaemontan.