蒲黄水煎剂对孕后期大鼠离体子宫平滑肌收缩活动的影响

Effects of Typhae pollen Decoction on Isolated Uterine Smooth Muscle Contraction of Rats at Postconceptual Stage

目的:研究蒲黄水煎剂对孕后期大鼠离体子宫平滑肌(USM)收缩活动的影响。方法:选取孕后期大鼠16只,取其USM肌条进行试验。累积加入终质量浓度为0(空白对照)、2、4、8、12 mg(生药)/ml的蒲黄水煎剂,观察其对USM肌条收缩活动的影响,检测并记录USM肌张力、收缩持续时间、收缩频率;并考察加入前列腺素合成酶抑制剂吲哚美辛(1×10^(-7)mol/ml)、钙离子通道阻滞药维拉帕米(1×10^(-7)mol/ml)预处理后蒲黄水煎剂[8 mg(生药)/ml]对离体USM收缩活动的影响,检测并记录USM肌张力、收缩持续时间、收缩频率。结果:与空白对照组比较,4、8、12 mg(生药)/ml蒲黄水煎剂均能使USM肌张力升高、收缩持续时间延长、收缩频率升高(P<0.05);加入维拉帕米和吲哚美辛后,蒲黄水煎剂对USM收缩作用影响减弱,使其肌张力降低、收缩持续时间缩短、收缩频率降低(P<0.05)。结论:蒲黄水煎剂能增强孕后期大鼠离体USM的收缩活动,且该作用可被前列腺素合成酶抑制剂与钙离子通道阻滞药抑制。

OBJECTIVE：To study the effect of Typhae pollen decoction on isolated uterine smooth muscle（USM）contraction of rats at postconceptual stage. METHODS：16 Wistar rats at postconceptual stage were selected and USM strips were isolated. T.pollen decoction 0（blank control）,2,4,8 and 12 mg（medicinal material）/ml were added respectively;the effects of T. pollen decoction on USM strips contraction were observed,and mean tension,duration of contraction and frequency were measured and recorded. The effects of T. pollen decoction on USM strips contraction were investigated after pretreatment of prostaglandin synthase inhibitor indometacin（1×10^-7mol/ml）and calcium channel blocker verapamil（1×10^-7mol/ml）;USM tension,duration of contraction and frequency were measured and recorded. RESULTS：Compared with blank control group,T. pollen decoction 4,8 and 12mg（medicinal material）/ml could increase USM tension,duration of contraction and frequency（P〈0.05）;after added verapamil and indometacin,T. pollen decoction decreased USM tension,shortened duration of contraction and decreased frequency（P〈0.05）. CONCLUSIONS：T. pollen decoction can strengthen USM strips contraction,and the effect is inhibited by prostaglandin synthase inhibitor and calcium channel blocker.