茉莉酸甲酯对牛膝生长及主要药用成分积累的影响

Effects of methy jasmonate on growth and major medicinal components accumulation of Achyranthes bidentata

不同浓度的外源茉莉酸甲酯(Me JA)对牛膝生长及其主要药用成分齐墩果酸和蜕皮甾酮均有不同的影响。该文采用不同浓度的Me JA分别浸种处理牛膝种子:0(对照)、0.1 mmol·L-1(T1)、0.15 mmol·L-1(T2)、0.2 mmol·L-1(T3)、0.25 mmol·L-1(T4),对照(CK)以同体积的蒸馏水浸泡。取浸种处理后生长培养60 d的牛膝植株,测定牛膝的株高、根长、地上及地下部分的生物量;取牛膝的叶及根,HPLC法测定其齐墩果酸和蜕皮甾酮的含量,研究Me JA对牛膝生长及主要药用成分齐墩果酸和蜕皮甾酮积累的影响。结果表明:0.15 mmol·L-1Me JA浸种处理,对牛膝生长及生物量的促进作用最佳,其株高、根长、地上部分鲜重及根鲜重分别比对照显著升高43.9%、38.7%、26.4%、64.0%(P<0.05);0.15 mmol·L-1Me JA处理,对牛膝的根和叶中齐墩果酸的积累作用最佳,分别比对照组显著增加了114.3%和60%(P<0.05);0.25 mmol·L-1Me JA处理,对牛膝根中蜕皮甾酮的积累最佳,比对照高出90.3%(P<0.05),却不利于根和叶中齐墩果酸的积累,并可抑制叶中蜕皮甾酮的形成。说明0.15 mmol·L-1Me JA浸种处理对牛膝的生长及其根和叶中齐墩果酸的积累作用最佳,并能显著促进根中蜕皮甾酮的积累,有利于牛膝药材产量和品质的提高。

To study the effects of methy jasmonate on the growth and the major medicinal components（ oleanolic acid and ecdysterone） accumulation of Achyranthes bidentata,seeds of A. bidentata were soaked for 3 h by different concentrations（ 0,0. 1,0. 15,0. 2,0. 25 mmol·L-1） of methy jasmonate,and the control groups were soaked in the same volume of distilled water in this study. The determination of plant height,root length,fresh weight of aboveground and root of A. bidentata were done after 60 d. The contents of ecdysterone and oleanolic acid in roots and leaves of A.bidentata were determined by HPLC. The results showed that the 0. 15 mmol·L-1Me JA treatment sample was benefitial for plant height,root length,fresh weight of aboveground and root. Compared with control,the biomass respectively be increased（ 43. 9%,38. 7%,26. 4%,64. 0%,P〈0. 05） observably. The 0. 15 mmol·L-1Me JA treatment sample was also benefitial for oleanolic acid accumulation in roots and leaves of A. bidentata. It had the highest amount respectively（ 114. 3% and 60%,P〈0. 05）. The 0. 25 mmol·L-1Me JA treatment sample was significantly promoted ecdysterone accumulation in roots,but inhibited oleanolic acid accumulation in roots and leaves and ecdysterone in leaves of A.bidentata. These results suggested that 0. 15 mmol · L-1Me JA soaking was benefitial for the growth,oleanolic acid accumulation in roots and leaves,and would significantly promote ecdysterone accumulation in roots of A. bidentata,and be helpful to improve the quality and yield of medicinal materials.