FISH检测痰脱落细胞3、8、9、17染色体异常情况,研究肺癌诊断新手段

Research on a New Mean for the Diagnosis of Lung Cancer:Fluorescence in Situ Hybridization in the Detection of Abnormalities of Chromosome 3,8,9,17 in Exfoliated Cells of Sputum Samples

目的:应用荧光原位杂交(fluorescence in situ hybridization,FISH)技术对痰脱落细胞染色体异常情况进行分析,探讨FISH辅助诊断肺癌的可行性和有效性。方法:选择2014年6月-2015年6月本院收治的疑似肺癌患者30例作为研究对象,采用3、8、9、17号染色体着丝粒探针对其痰脱落细胞进行FISH检测并进行痰脱落细胞学检查。结果:肺癌确诊患者痰脱落细胞中3、8、9、17号染色体畸变阳性率分别为41.67%、45.83%、58.33%、41.67%;FISH检测和痰脱落细胞学检查的敏感度分别为83.33%和20.83%,特异性分别为66.67%和100%,诊断符合率分别为80.00%和36.67%,FISH检测敏感度和诊断符合率均高于痰脱落细胞学检查,比较差异均有统计学意义(P<0.05)。结论:肺癌的发生发展与染色体畸变有关,FISH检查可明显提高肺癌诊断准确率,可作为肺癌诊断新手段。

Objective：To analyze the chromosome abnormalities of exfoliated cells in sputum samples by fluorescence in situ hybridization and to explore the possibility and validity of FISH examination for the diagnosis of lung cancer.Method：30 patients who were suspected with lung cancer in our hospital from June 2014 to June 2015 were selected as the research objects.They were given FISH examination by using centromeric probes of chromosome 3,8,9,17 to examine chromosome abnormalities of sputum exfoliated cells,sputum cytology detection was also given to them.Result：The aberration rate of chromosomes 3,8,9 and 17 in lung cancer patients＇ sputum exfoliated cells were 41.67%,45.83%,58.33% and 41.67% respectively.The sensitivity of FISH and sputum cytology detection were 83.33% and 20.83% respectively,the specificity of them were 66.67% and 100% respectively and the diagnostic accordance rate of them were 80.00% and 36.67% respectively.The sensitivity and the diagnostic accordance rate of the FISH examination were higher than those of the sputum cytology detection,the differences were statistically significant（P〈0.05）.Conclusion：Chromosome aberrations plays an important role in the development of lung cancer.FISH technique can significantly improve the accuracy rate of diagnosis for lung cancer.It can be used as a new mean for the diagnosis of lung cancer.