大鼠脊髓损伤进行自体神经移植后荧光金逆行示踪观察

Retrograde tracing of fluorescent gold after autogenous nerve transplantation on spinal cord injured in rats

目的探讨大鼠脊髓损伤后使用自体腓肠神经组织移植修复后的荧光金逆行示踪变化。方法将实验动物分为自体腓肠神经移植组和非移植组两组,每组各18例。利用改良Allen撞击方法建立脊髓损伤模型,损伤4周后,移植组利用自体腓肠神经移植修复脊髓损伤段,非移植组仅暴露切口不做处理。分别于术后4、6、8周,行荧光金(fluoro gold,FG)逆行示踪了解脊髓损伤轴浆运输恢复情况。结果移植组术后4周,FG阳性标记神经元稀少;移植术后6周,FG阳性细胞逐渐增多;移植术后8周,FG阳性细胞密集。与非移植组各组相比,差异有统计学意义(P<0.01)。结论大鼠脊髓损伤后自体神经移植修复,存活良好并促进脊髓损伤段轴浆运输功能恢复。

Objective To investigate the changes of the fluoro gold （FG） retrograde tracing autogenous nerve transplantation on spinal cord injured in rats. Methods The animals were divided into two groups, with modified Allen impact method to establish model of spinal cord injury. After four weeks, in the transplantation group, autologous sural nerve graft was used to repair spinal cord injury period and in non-transplantation group was only exposed incision without treatment. In the four, six and eight weeks after operation, the retrograde tracing of FG was performed to discover the recovery of the axial plasma trans- port. Results FG positive labeled neurons were sparse in four weeks. At six weeks after transplantation, FG positive cells were gradually increased, and the FG positive cells were concentrated in eight weeks after transplantation. Compared with the control group, there were significant differences （P〈0.01）. Conclusion After spinal cord injury, autologous nerve graft is repaired and survived well and promote the recovery of spinal cord injury segment shaft pulp transportation function.