甘肃金银花(盘叶忍冬)抗菌作用实验研究

Experimental Study on Antibacterial Effects of Gansu Honeysuckle

目的:观察甘肃金银花(盘叶忍冬)对金黄色葡萄球菌和大肠埃希菌的体内、外抗菌作用。方法:体内抗菌用金黄色葡萄球菌和大肠埃希菌感染小鼠,观测给药小鼠在染菌后7天内的死亡率;体外抗菌用琼脂扩散实验和菌落计数法测定最小抑菌浓度(MIC)和抑菌圈大小。结果:对感染金黄色葡萄球菌和大肠埃希菌小鼠灌胃甘肃金银花(盘叶忍冬)后小鼠的死亡率分别为45%和40%;正品金银花为40%;两者与模型对照组小鼠死亡率的95%相比,差异有统计学意义(P<0.05)。体外抗菌实验测得甘肃金银花(盘叶忍冬)和正品金银花对金黄色葡萄球菌和大肠埃希菌的最小抑菌浓度为6.25 mg/m L和12.5 mg/m L。结论:甘肃金银花(盘叶忍冬)对金黄色葡萄球菌和大肠埃希菌具有一定的体、内外抗菌作用。

Objective： To observe antibacterial effects of Gansu honeysuckle （Lonicera tragophylla Hemsl.） on Staphylococcus aureus （SA） and E.coli in vivo and vitro. Methods： Mortality rate of the medicated mice were detect- ed in seven days after the mice were infected by SA and E.coli; minimal inhibitory concentration （MIC） and the size of inhibition zone were measured through agar diffusion experiments and bacterial colony counting method. Results： SA-infected and E.coli-infected mice were treated by Gansu honeysuckle and genuine honeysuckle, mortality rates of SA-infected and E.coli-infected mice treated by Gansu honeysuckle were 45% and 40% respectively; mortality rate of genuine honeysuckle was 40%; the difference was significant when both were compared with model control group with the mortality rate 95%（P〈 0.05）. The results of antibacterial test showed that MIC of Gansu honeysuckle and genuine honeysuckle against SA and E.coli were 6.25 and 12.5 mg/mL. Conclusion： Gansu honeysuckle shows antibacterial effects to SA and E.coli in vivo and vitro to a certain degree.