栽培基质和营养液浓度对白发藓生长影响的研究

On influences of cultivated media and nutrient concentrations on growth of leucobryum glaucum

通过对白发藓生长最佳基质和营养液的筛选,来大量纯种扩繁白发藓.通过组织培养得到了白发藓(Leucobryumglaucum)的无菌配子体,将它们接种于装有泥炭土、蛭石和珍珠岩不同基质比例的培养瓶内,并加入不同浓度的knop's营养液,通过60 d的培养,测定了不同处理的白发藓配子体的叶绿素含量,配子体长度、总鲜重和分枝数等指标.发现泥炭土∶蛭石∶珍珠岩2∶2∶1比例、20%浓度的Knop's营养液有利于白发藓配子体的生长.

Screening by Leucobryum glaucum growth optimal substrate and nutrient solution,to a large number of purebred breeding leucobryum. The flask culture conditions of the gametophyte of Leucobryum glaucum inoculated with peat,vermiculite and perlite matrix at different proportions,and adding knop＇ s nutrition liquid at different concentrations. After a 60-day cultivation,the chlorophyll content,length,total fresh weight and branch number of the gametophytes of L. glaucum were measured. The results showed that L. glaucum could grow well on the medium with a proportion of peat,vermiculite and perlite at 2∶ 2∶ 1,together with20% Knop＇s nutrition liquid.