侵染油茶的两种病原菌的鉴定及其生物学特性研究

The Study of Two Camellia Pathogens Isolation,Identification and Biological Characteristics

为了掌握湖北孝感本地油茶(Camellia oleifera)主要病害发生规律,采用常规组织分离法分离病原菌,依据病原菌的形态特征,结合其r DNA-ITS序列分析,对油茶病原菌进行分离和分子鉴定,并对其生物学特性进行了研究。结果表明,鉴定两种病原菌分别为胶孢炭疽菌(Colletotrichum gloeosporioides)和链格孢(Alternaria alternata);炭疽病菌菌株在PDA培养基中生长最快,最适碳源和氮源分别为淀粉、硫酸铵,p H 6.0为宜,最适生长温度为25℃,致死温度为52℃;链格孢菌株最适培养基为PDA培养基,以淀粉为碳源、硝酸铵为氮源为宜,p H 8.0时菌丝生长最快,最适温度为25℃,致死温度为54℃。

The two Camellia pathogens were isolated, identified, and the primary biological characteristics were determined.By using routine isolation method from tissue, following morphological characteristics, combining with the result of the r DNAITS sequence analysis, the two pathogens were identified as Colletotrichum gloeosporioides and Alternaria alternata,respectively.The test showed the pathogen C. gloeosporioides grew best in PDA media,starch as carbon source,ammonium sulfate as nitrogen source,p H 6.0, the optimum temperature was 25 ℃ and the lethal temperature was 52 ℃;For A. alternata,which grew best in CMM, starch as the carbon source,ammonium nitrate as nitrogen source,p H 8.0,the optimum temperature was 25 ℃,and the lethal temperature was 54 ℃.