腹腔注射四氯化碳建立大鼠肝纤维化模型

Studies on Rats Hepatic Fibrosis Model Established by Intraperitoneal Injection of Carbon Tetrachloride

腹腔注射四氯化碳（Carbon Tetrachloride,CCl4）构建大鼠肝纤维化模型,追踪观察模型大鼠病理组织学改变,为临床研究提供可靠的实验模型。模型组雄性Sprague-Dawley（SD）大鼠按1.0 ml/kg剂量腹腔注射40%CCl4-橄榄油混悬液,每周3次,共计8周;对照组同时腹腔注射等量的生理盐水。于给药1-4、6、8周末每组随机取6只大鼠肝脏,肉眼观察肝脏组织大体改变,经HE和Masson染色观察肝脏病理学改变。肝脏组织光镜下显示：模型组给药第2周肝细胞发生脂肪变性;第4周肝组织局部形成假小叶,门管区有大量纤维细胞聚集;第6-8周肝组织出现大面积假小叶,建模成功率高达88.3%。采用多点、密集的腹腔注射方法可更有效地建立大鼠肝纤维化模型,为临床提供可靠的研究模型。

To establish rat liver fibrosis model,CCl_4 solution was injected intraperitoneally and the pathological changes were observed. The result can offer fibrosis model for clinical studies. The authors use SD male rats as model group,and they were injected 40% CCl_4-olive oil solution,and the control group was injected saline,intraperitoneally three times a week for 8 weeks. At the end of the 1st,2nd,3rd,4th,6th,and 8th weeks,6 rats from each group were randomly selected to demonstrate HE and Masson staining and observe the liver pathological changes. Hepatic steatosis was observed under the microscopy after CCl4-olive oil administration for 2 weeks in model group. After 4 weeks,some pseudolobules formed and numerous fibroblasts assembled at the portal area. At six to eight weeks,numerous liver pseudolobules appeared. The rate of success modeling is up to 88. 3%. Hence,multi-point and intensive intraperitoneal injection method can be more effective to rat liver fibrosis model,which is reliable for clinical research.