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中药葛根微生物转化菌株的鉴定及转化特性的研究 被引量:3

Study on Identification of the Microbial Strain for Transforming Puerariae Radix and Its Microbial Characteristics
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摘要 从中药葛根的微生物转化试验中,获得1株能够较好转化葛根有效成分的丝状真菌,编号为YM3207,通过对该菌的形态特征观察及ITS序列分析,确定该菌为紫色红曲霉Monascus purpureus。经HPLC定量分析显示,转化后染料木素和葛根素含量得到提高;同时,抑菌活性较转化前有一定程度的增强,抗菌谱变广。 A strain of filamentous fungi which was able to transform the active ingredient in Puerariae radix was obtained in microbialtransformation experiments and named YM3207. After observing and analyzing the morphology and ITS sequence of this fungi, it wasidentified as Monascus purpureus. HPLC was used to analyze the change of transformation column. The content of Genistein andPuerarin have been improved. At the same time, antibacterial activity enhanced and antimicrobial spectrum expanded as well.
出处 《大理学院学报(综合版)》 CAS 2015年第12期64-68,共5页 Journal of Dali University
关键词 葛根 微生物转化 鉴定 抑菌 Puerariae radix microbial transformation identification antibacterial
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参考文献15

  • 1LOUGHLIN W A. Biotransformation in organic synthesis[J]. Bioresource Technol, 2000 (74) : 49-62.
  • 2占纪勋,钟建江,戴均贵,郭洪祝,朱蔚华,张元兴,果德安.红豆杉愈伤组织中紫杉烷类成分sinenxan A的微生物转化研究[J].药学学报,2003,38(7):555-558. 被引量:17
  • 3唐燕红,张日俊.微生物在中草药添加剂中的应用研究进展[J].中国饲料,2004(12):20-21. 被引量:11
  • 4肖培根.新编中药志:卷1[M].北京:化学工业出版社,2001:962-974.
  • 5范秀容 李广武 沈萍.微生物学实验[M].北京:高等教育出版社,1989.5.
  • 6李绍兰,周斌,杨丽源,李治滢,张琦,陈有为.真菌DNA提取方法的改良[J].云南大学学报(自然科学版),2002,24(6):471-472. 被引量:43
  • 7THOMPSON J D, GIBSON T J, PLEWNIAK F, et al. The Clastal X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools [J].Nucleic Acids Research, 1997 (24) : 4876-4882.
  • 8JEANNMOUGIN F, THOMPSON J D, GOUY M, et al. Multiple sequence alignment with Clustal X [J]. Trends Biochemistry Science, 1998 (23) : 403-405.
  • 9KUMAR S, TAMURA K, JAKOBSEN I B, et al. MEGA2: Moleeualr revolutionary genetics analysis software [J]. Bioinformatics ,2001 (17) : 1244-1245.
  • 10COMERON J M. A method for estimating the numbers of synonymous and nonsynonymous substitutions per site [J]. Journal of Molecular Evolution, 1995 (41 ) : 1152-1159.

二级参考文献23

  • 1吴定,江汉湖.高蛋白发酵食品丹贝研究进展[J].食品与发酵工业,1994,20(3):72-74. 被引量:22
  • 2张蕾,朱蓉贞,潘扬,马国祥.不同品种及产地的葛根中葛根素含量的比较[J].中国中药杂志,1995,20(7):399-400. 被引量:29
  • 3章育中 等.高效液相色谱法测定葛根及其片剂中异黄酮的含量[J].药物分析杂志,1984,4(2):67-67.
  • 4周德庆.微生物学实验手册[M].上海:上:海科学技术出版社,1987..
  • 5马绪荣 苏德模.药品微生物学检验手册[M].北京:科学出版社,2001.106-121.
  • 6Kudou S, Shimoyamada M, Imura t. a new isoflavone glycoside in soybean seeds (Glycine max Merrill), glycitein 7 -0 - 0634 - D -(6"-0- acetyl)- glucopyranoside. J Agric Biol Chem,1991,55:859 - 860.
  • 7Kudou S, Fleury Y, Welti D. Malonyl isoflavone glycosides in soybean seeds (Glycine max Merrill). J Agric Biol Chem,1991,55:2227 - 2233.
  • 8Huang A S, Hsieh O A L and Chang S S. Characterization of the nonvolatile minor constituents responsible for the objectionable taste of defatted soybean flour. J Food Sci , 1981,29:19-23.
  • 9Coward L, Bames N C, Kenneth D R et al. Genistein, daidzein and their β - glucoside conjugates : antitumor isoflavones in soybean foods from American and Asian diets. J Agric Food Chem,1993,41 ( 11 ) : 1961 - 1967.
  • 10Naim M, Gestetner B, Zikah S et al. Soybean isoflavones. Characterization, determination and antifungal activity. J Agric Food Chem, 1974,22:806-810.

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