p53和Ki67在急性白血病中的表达及其临床意义

Clinical significance and expression of p53 and Ki67 in acute leukemia

目的探讨急性白血病患者细胞中p53和Ki67的表达,并对其在治疗和预后中的作用进行分析。方法收集30例非血液恶性病患者和38例急性白血病患者的骨髓或外周血,采用流式细胞仪检测细胞中p53和Ki67的表达水平,然后进行统计学分析。结果与对照组相比较,急性白血病组患者的p53和Ki67阳性率明显增高,且p53和Ki67表达水平与患者的年龄、血红蛋白、外周血白细胞总数、骨髓原始细胞比例及血小板计数无相关性。结论急性白血病患者细胞中p53和Ki67高表达,将二者联合起来检测,不但可深入了解病情,同时也是观察疗效和判断预后的有效指标之一。

Objective To investigate the expression of p53 and Ki67 from the acute leukemia cells,and their role in the treatment and prognosis were analyzed. Methods Totally 30 cases of hematological malignant disease and 38 cases of patients with acute leukemia were collected,and the expression of p53 and Ki67 level in bone marrow or peripheral blood were tested by flow cytometry instrument,and then performed statistical analysis. Results Compared with control group,p53 and Ki67 positive rate of patients with acute leukemia group increased obviously,and p53 and Ki67 expression level had no correlation with the patient＇s age,hemoglobin,total number of peripheral blood leukocytes,primitive bone marrow cells and platelet count. Conclusion p53 and Ki67 highly expressed in patients with acute leukemia cells,and the two combined to detect not only can understand the illness,but also was one of the effective indicators to observe curative effect and prognosis.