成骨细胞中Caspase蛋白和Bcl-2蛋白在绝经后骨质疏松症发病机理中的作用

Effects of Caspase proteins and Bcl-2 protein of osteoblasts on pathogenesis of postmenopausal osteoporosis

目的探讨半胱氨酸天冬氨酸蛋白酶(Caspase)-3、Caspase-9、B细胞淋巴瘤-2(Bcl-2)蛋白对绝经后骨质疏松症发病机理的影响。方法 2012年1月至2012年12月期间收集广州医科大学附属第一医院老年病科住院的需行髋关节置换手术的女性绝经期患者20例,分为骨质疏松症组与对照组,每组各10例。术中取下股骨头或股骨颈中松质骨,分别进行成骨细胞的体外培养,通过酶联免疫吸附试验(ELISA)方法检测两组患者成骨细胞中Caspase-3、Caspase-9、Bcl-2蛋白表达情况。两独立样本均数比较采用t检验,方差不齐的采用秩和检验。结果与正常对照组比较,骨质疏松症组患者的成骨细胞中Caspase-3(11.0±1.5 pmol/L)、Caspase-9(10.9±1.7 pmol/L)的表达水平均较高,差异均有统计学意义(Caspase-3:t=5.76,P<0.05;Caspase-9:t=4.47,P<0.05);而骨质疏松症组患者的成骨细胞中Bcl-2(1.3±0.5 ng/ml)低于正常对照组,差异有统计学意义(t=2.43,P<0.05)。结论在骨质疏松症的成骨细胞凋亡过程中,Caspase依赖性凋亡途径激活,而Bcl-2途径受到抑制,二者可能参与了绝境后骨质疏松症的发病机理。

Objective To investigate the effects of Caspase-3,Caspase-9,B-cell lymphoma-2（ Bcl-2） protein in the pathogenesis of postmenopausal osteoporosis. Methods From January 2012 to December 2012,20 cases of hip replacement surgery in the First Affiliated Hospital of Guangzhou Medical University were enrolled. All the patients who were female and in menopause period were divided into the osteoporosis group（ n = 10） and the control group（ n = 10）. The cancellous bones in the femoral head and femoral neck were collected during the hip replacement and were used to culture osteoblasts. Caspase-3,Caspase-9 and Bcl-2 were examined using enzyme-linked immunosorbent assay（ ELISA）. Two independent samples were compared using t test,if variance unequal,the samples were compared by rank-sum test.Results Compared with the control group,the expression levels of Caspase-3 and Caspase-9 increased and the expression level of Bcl-2 obviously decreased in the osteoporosis group [Caspase-3（ 11. 0 ± 1. 5） pmol /L,Caspase-9（ 10. 9 ± 1. 7） pmol / L,Bcl-2（ 1. 3 ± 0. 5） ng / ml]; the differences between the two groups were statistically significant（ Caspase-3： t = 5. 76,P〈0. 05; Caspase-9： t = 4. 47,P〈0. 05; Bcl-2： t =2. 43,P〈0. 05）. Conclusion In the process of osteoblast apoptosis in osteoporosis,Caspases-dependent apoptotic pathways may be activated,and the Bcl-2 way is restrained,which may be involved in the pathogenesis of the postmenopausal osteoporosis.