秋水仙素诱导非洲凤仙多倍体的研究

Polyploid Induction in Impatiens walleriana Using Colchicine

以非洲凤仙扦插苗为试材,采用不同浓度(0.2%和0.5%)秋水仙素溶液,在不同处理时间(48h和72h)下诱导非洲凤仙扦插苗,通过其形态观察及染色体计数进行倍性鉴定。结果表明:秋水仙素诱导非洲凤仙得到了四倍体,在0.5%浓度,48h条件下诱导效果最好。二倍体非洲凤仙染色体数目为2n=2x=16,诱导的同源四倍体染色体数目为2n=4x=32。四倍体与二倍体在形态学和细胞学上具有明显的差异。

The effects of colchicine concentration（0.2%,0.5%）and treatment time（48h,72h）duration on the apical meristems of the seedlings in I.walleriana has been conducted in this study,and the ploidy level was identified by morphological trait,stoma size and chromosome number.Results：Treated I.walleriana with 0.5% colchicine for 48 hlead to the highest mutation rate,a number of tetraploidy plants were obtained.The choromosome number of mutated plants was 2n=4x=32,while that of the control plants was 2n=2x=16.The tetraploid plants exibited some morphological and cytological variations.