红景天提取物体外抗肿瘤转移作用机制研究

Mechanism of anti-tumor metastasis for Rhodiola extraction in vitro

目的:研究红景天提取物体外抗肿瘤转移作用机制。方法:采用MTT、划痕实验、matrigel侵袭实验、血管生成实验及Western blot蛋白免疫印迹实验分别检测红景天提取物不同浓度对人乳腺癌细胞MDA-MB-231及人脐静脉内皮细胞(HUVEC)的细胞毒作用、侵袭能力、迁移能力和血管生成能力的影响。结果:红景天提取物40 mg/ml对人乳腺癌细胞MDA-MB-231及HUVEC均无毒性,在不同浓度下均能抑制人乳腺癌细胞MDA-MB-231的侵袭、迁移能力及HUVEC血管生成能力,并能显著性抑制PI3K/Akt信号通路Akt及PKCζ蛋白磷酸化水平。结论:红景天提取物能够从抑制肿瘤细胞侵袭、迁移及血管生成多方面发挥抗肿瘤转移作用,并通过抑制PI3K/Akt信号通路抑制肿瘤细胞的转移。

Objective： To study the mechanism of anti- tumor metastasis for Rhodiola extraction in vitro. Methods： The effects of Rhodiola extraction on cytotoxicity,cell migration,invasion,tube formation and the phosphorylation of protein of human breast cancer cell（ MDA- MB- 231） and human umbilical vein endothelial cell（ HUVEC） were measured by MTT assay,wound healing assay,invasion assay,tube formation assay and western blot assay,respectively. Results： Rhodiola extraction exhibited no cytotoxicity for MDA- MB- 231 and HUVEC cells at 40 mg / ml,and inhibited cell migration,invasion and tube formation with various concentrations. Furthermore,Rhodiola extraction also decreased phosphorylation of Akt and PKCζ on PI3 K / Akt signaling pathway significantly. Conclusion： Rhodiola extraction can suppress anti- tumor metastasis by inhibiting cancer cell migration,invasion,tube formation,and the PI3 k / Akt signaling pathway.