猴耳环化学成分的分离与鉴定

Isolation and identification of chemical constituents from Pithecellobium Clypearia Bemth

目的：对猴耳环嫩枝及叶70%乙醇-水提取物的化学成分进行分离鉴定。方法：利用多种色谱法对提取物进行分离,通过理化性质、波谱数据分析等方法,鉴定了化合物的结构。结果：从猴耳环嫩枝及叶70%的醇提物中分离到8个已知单体化合物（Ⅰ-Ⅷ）,光谱法鉴定结构分别为（＋）-儿茶素（Ⅰ）、（-）-（2S）-5,7,3＇,4＇,5＇-五羟基黄烷（Ⅱ）、（-）-（2R）-5,7,3＇,4＇,5＇-五羟基黄烷-7-没食子酸酯（Ⅲ）、（-）-（2R,3R）-表没食子儿茶素-3-O-没食子酸酯（Ⅳ）、3-（4＇-羟基-3＇,5＇-二甲氧基）-苯基-1,2-二丙醇（Ⅴ）、3-（4＇-羟基-3＇-甲氧基）-苯基-1,2-二丙醇（Ⅵ）、绿原酸乙酯（Ⅶ）、4-O-β-D-吡喃葡萄糖基反式肉桂酸（Ⅷ）。结论：化合物Ⅴ-Ⅷ为首次从该属植物中分离得到。

Objective： To study the chemical constituents of the extracts with 70% ethanol- water from the twigs and leaves of Pithecellobium Clypearia Bemth. Methods： The compounds were isolated with macroporous resin,polyamide,ODS,Sephadex LH- 20,HPLC,etc. their structures were identified by means of spectral data and literature. Results： The chemical structures of8 compounds were elucidated as（ ＋）- catechin（ I）,（-）-（ 2S）- 5,7,3＇,4＇,5＇- pentahydroxyflavan（ II）,（-）-（ 2R）- 5,7,3＇,4＇,5＇- pentahydroxyflavan（ III）,（-）-（ 2R,3R）- epigallocatechin- 3- O- gallate（ IV）,3-（ 4＇- hydroxy- 3＇,5＇- dimethoxyphenyl）- propane- 1,2- diol（ V）,3-（ 4＇- hydroxy- 3＇- methoxyphenyl）- propane- 1,2- diol（ VI）,chlorogenic acid ethyl ester（ VII）,4- O- β- D- Glucopyranosyl- trans- p- coumaric acid（ VIII）. Conclusion： Compounds V - VIII were isolated from this genus for the first time.